Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia

Bibliographic Details
Main Author: Marques, Ester Augusta Lima Vinhas
Publication Date: 2016
Other Authors: Silva, Norma Lucena Cavalcanti Licinio da, Pedrosa, Francisco de Paula Ramos
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1002/cyto.b.21394
Download full: https://arca.fiocruz.br/handle/icict/33204
Summary: Institute of Integral Medicine Professor Fernando Figueira. Centro de Hematologia e Oncologia Pediátrica. Pediatric Oncology. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
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spelling Marques, Ester Augusta Lima VinhasSilva, Norma Lucena Cavalcanti Licinio daPedrosa, Francisco de Paula Ramos2019-05-24T12:50:33Z2019-05-24T12:50:33Z2016-06-25MARQUES, Ester Augusta Lima Vinhas; SILVA, Norma Lucena Cavalcanti Licinio da; PEDROSA, Francisco de Paula Ramos. Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia. Cytometry Part B: Clinical Cytometry, v. 94, n. 1, p. 94-99, 25 June 2016.1552-4949https://arca.fiocruz.br/handle/icict/3320410.1002/cyto.b.213941552-4957engWileyImplementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitute of Integral Medicine Professor Fernando Figueira. Centro de Hematologia e Oncologia Pediátrica. Pediatric Oncology. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Institute of Integral Medicine Professor Fernando Figueira. Centro de Hematologia e Oncologia Pediátrica. Pediatric Oncology. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Institute of Integral Medicine Professor Fernando Figueira. Centro de Hematologia e Oncologia Pediátrica. Pediatric Oncology. Recife, PE, Brasil.Background: Monitoring minimal residual disease (MRD) in B-lineage acute lymphoblastic leukemia (ALL) to assess treatment response is crucial for risk assignment. Flow cytometry can be used to monitor MRD in ALL but the implementation of this approach requires extensive expertise. If resources are limited, the costs of full flow cytometric MRD testing might be prohibitive. Objective: We evaluated the applicability of a previously reported simplified MRD assay, designed to distinguish leukemic from normal lymphoblastic during remission induction therapy. Methods: Fifty-nine samples from children with ALL, enrolled in the RE-LLA study at a pediatric oncology center in Recife (Brazil), were evaluated for MRD on day 19 and on day 26 of remission induction therapy. We compared results obtained by a trainee in Recife and an expert. Results: The method was implemented successfully and the concordance between results obtained by the trainee and the expert was practically absolute at the end of the study. Conclusions: It is possible to implement reliable measurements of MRD during remission induction therapy in childhood ALL despite limited resources. The simplicity of the MRD method used in this study does not require extensive prior training in leukemia immunophenotypingAcute lymphoblastic leukemiaMinimal residual diseaseFlow cytometryAdolescenteBrasilCriançaCriança, pré-escolarFêmeaCitometria de FluxoHumanosInfantilRecém-NascidoLinfócitosMasculinoNeoplasia ResidualLeucemia Linfoma Linfoblástica de Células Precursoras03 Saúde e Bem-Estar09 Indústria, inovação e infraestrutura10 Redução das desigualdadesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83094https://arca.fiocruz.br/bitstreams/8df618d9-b43d-48de-a3fd-8bf2ee872573/downloadee3692f7b0b97d5638099ab94397d05fMD51falseAnonymousREADORIGINALVinhas_et_al-2018-Cytometry_Part_B__Clinical_Cytometry.pdfVinhas_et_al-2018-Cytometry_Part_B__Clinical_Cytometry.pdfapplication/pdf189154https://arca.fiocruz.br/bitstreams/0b6cddfb-4ef9-4f16-8711-a5b0b873709d/download5edf73d89f1339d6e0a2be99d6791ba2MD52trueAnonymousREADTEXTVinhas_et_al-2018-Cytometry_Part_B__Clinical_Cytometry.pdf.txtVinhas_et_al-2018-Cytometry_Part_B__Clinical_Cytometry.pdf.txtExtracted texttext/plain24663https://arca.fiocruz.br/bitstreams/449cd65f-1bff-45da-916e-1bd8351ad647/download5505a7fa19e4927a680058c230b0802eMD55falseAnonymousREADTHUMBNAILVinhas_et_al-2018-Cytometry_Part_B__Clinical_Cytometry.pdf.jpgVinhas_et_al-2018-Cytometry_Part_B__Clinical_Cytometry.pdf.jpgGenerated Thumbnailimage/jpeg5964https://arca.fiocruz.br/bitstreams/b0fc8322-0ce8-4ce0-a678-759266dc85bb/download1120a469ea87380b4ffcebac21d40af2MD56falseAnonymousREADicict/332042025-08-04 13:41:35.776open.accessoai:arca.fiocruz.br:icict/33204https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-08-04T16:41:35Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
title Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
spellingShingle Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
Marques, Ester Augusta Lima Vinhas
Acute lymphoblastic leukemia
Minimal residual disease
Flow cytometry
Adolescente
Brasil
Criança
Criança, pré-escolar
Fêmea
Citometria de Fluxo
Humanos
Infantil
Recém-Nascido
Linfócitos
Masculino
Neoplasia Residual
Leucemia Linfoma Linfoblástica de Células Precursoras
03 Saúde e Bem-Estar
09 Indústria, inovação e infraestrutura
10 Redução das desigualdades
title_short Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
title_full Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
title_fullStr Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
title_full_unstemmed Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
title_sort Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia
author Marques, Ester Augusta Lima Vinhas
author_facet Marques, Ester Augusta Lima Vinhas
Silva, Norma Lucena Cavalcanti Licinio da
Pedrosa, Francisco de Paula Ramos
author_role author
author2 Silva, Norma Lucena Cavalcanti Licinio da
Pedrosa, Francisco de Paula Ramos
author2_role author
author
dc.contributor.author.fl_str_mv Marques, Ester Augusta Lima Vinhas
Silva, Norma Lucena Cavalcanti Licinio da
Pedrosa, Francisco de Paula Ramos
dc.subject.en.none.fl_str_mv Acute lymphoblastic leukemia
Minimal residual disease
Flow cytometry
topic Acute lymphoblastic leukemia
Minimal residual disease
Flow cytometry
Adolescente
Brasil
Criança
Criança, pré-escolar
Fêmea
Citometria de Fluxo
Humanos
Infantil
Recém-Nascido
Linfócitos
Masculino
Neoplasia Residual
Leucemia Linfoma Linfoblástica de Células Precursoras
03 Saúde e Bem-Estar
09 Indústria, inovação e infraestrutura
10 Redução das desigualdades
dc.subject.decs.none.fl_str_mv Adolescente
Brasil
Criança
Criança, pré-escolar
Fêmea
Citometria de Fluxo
Humanos
Infantil
Recém-Nascido
Linfócitos
Masculino
Neoplasia Residual
Leucemia Linfoma Linfoblástica de Células Precursoras
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
09 Indústria, inovação e infraestrutura
10 Redução das desigualdades
description Institute of Integral Medicine Professor Fernando Figueira. Centro de Hematologia e Oncologia Pediátrica. Pediatric Oncology. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016-06-25
dc.date.accessioned.fl_str_mv 2019-05-24T12:50:33Z
dc.date.available.fl_str_mv 2019-05-24T12:50:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARQUES, Ester Augusta Lima Vinhas; SILVA, Norma Lucena Cavalcanti Licinio da; PEDROSA, Francisco de Paula Ramos. Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia. Cytometry Part B: Clinical Cytometry, v. 94, n. 1, p. 94-99, 25 June 2016.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/33204
dc.identifier.issn.none.fl_str_mv 1552-4949
dc.identifier.doi.none.fl_str_mv 10.1002/cyto.b.21394
dc.identifier.eissn.none.fl_str_mv 1552-4957
identifier_str_mv MARQUES, Ester Augusta Lima Vinhas; SILVA, Norma Lucena Cavalcanti Licinio da; PEDROSA, Francisco de Paula Ramos. Implementation of a simplified flow cytometric assays for minimal residual disease monitoring in childhood acute lymphoblastic leukemia. Cytometry Part B: Clinical Cytometry, v. 94, n. 1, p. 94-99, 25 June 2016.
1552-4949
10.1002/cyto.b.21394
1552-4957
url https://arca.fiocruz.br/handle/icict/33204
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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