An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures
| Main Author: | |
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| Publication Date: | 2015 |
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| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.vaccine.2015.03.077 |
| Download full: | https://arca.fiocruz.br/handle/icict/32037 |
Summary: | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil |
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Pereira, Renata C.Silva, Andrea N. M. R.Souza, Marta Cristina O.Silva, Marlon V.Neves, Patrícia P. C. C.Silva, Andrea A. M. V.Matos, Denise D. C. S.Herrera, Miguel A. O.Yamamura, Anna M. Y.Freire, Marcos S.Gaspar, Luciane P.Caride, Elena2019-03-12T13:33:41Z2019-03-12T13:33:41Z2015PEREIRA, Renata C. et al. An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures. Vaccine, Amsterdam, v. 33, n. 35, p. 4261-4268, 2015.1873-2518https://arca.fiocruz.br/handle/icict/3203710.1016/j.vaccine.2015.03.077engAn inactivated yellow fever 17DD vaccine cultivated in Vero cell culturesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilYellow fever is an acute infectious disease caused by prototype virus of the genus Flavivirus. It is endemic in Africa and South America where it represents a serious public health problem causing epidemics of hemorrhagic fever with mortality rates ranging from 20% to 50%. There is no available antiviral therapy and vaccination is the primary method of disease control. Although the attenuated vaccines for yellow fever show safety and efficacy it became necessary to develop a new yellow fever vaccine due to the occurrence of rare serious adverse events, which include visceral and neurotropic diseases. The new inactivated vaccine should be safer and effective as the existing attenuated one. In the present study, the immunogenicity of an inactivated 17DD vaccine in C57BL/6 mice was evaluated. The yellow fever virus was produced by cultivation of Vero cells in bioreactors, inactivated with β-propiolactone, and adsorbed to aluminum hydroxide (alum). Mice were inoculated with inactivated 17DD vaccine containing alum adjuvant and followed by intracerebral challenge with 17DD virus. The results showed that animals receiving 3 doses of the inactivated vaccine (2 μg/dose) with alum adjuvant had neutralizing antibody titers above the cut-off of PRNT50 (Plaque Reduction Neutralization Test). In addition, animals immunized with inactivated vaccine showed survival rate of 100% after the challenge as well as animals immunized with commercial attenuated 17DD vaccine.Inactivated vaccineProtective responseYellow fever03 Saúde e Bem-Estarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://arca.fiocruz.br/bitstreams/ed898e65-55e6-442a-abd8-e805b007d3e5/download8a4605be74aa9ea9d79846c1fba20a33MD51falseAnonymousREADORIGINAL1-s2.0-S0264410X15003965-main.pdf1-s2.0-S0264410X15003965-main.pdfapplication/pdf957405https://arca.fiocruz.br/bitstreams/75bfc561-6649-494d-a756-e813c1b40919/download51e928d5e63ef04d9fda9967ba002bedMD52trueAnonymousREADTEXT1-s2.0-S0264410X15003965-main.pdf.txt1-s2.0-S0264410X15003965-main.pdf.txtExtracted texttext/plain100333https://arca.fiocruz.br/bitstreams/d24148c0-0e5f-4013-a92c-eeb2301f8af9/download7277009c3c1feb31a099daada9357cd0MD57falseAnonymousREADTHUMBNAIL1-s2.0-S0264410X15003965-main.pdf.jpg1-s2.0-S0264410X15003965-main.pdf.jpgGenerated Thumbnailimage/jpeg5854https://arca.fiocruz.br/bitstreams/b201040d-09c4-44bf-89b2-e73dfb9f1116/download7920680fc2c0503d3387ff514a6570ddMD58falseAnonymousREADicict/320372025-07-29 18:48:17.937open.accessoai:arca.fiocruz.br:icict/32037https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T21:48:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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 |
| dc.title.none.fl_str_mv |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| title |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| spellingShingle |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures Pereira, Renata C. Inactivated vaccine Protective response Yellow fever 03 Saúde e Bem-Estar |
| title_short |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| title_full |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| title_fullStr |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| title_full_unstemmed |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| title_sort |
An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures |
| author |
Pereira, Renata C. |
| author_facet |
Pereira, Renata C. Silva, Andrea N. M. R. Souza, Marta Cristina O. Silva, Marlon V. Neves, Patrícia P. C. C. Silva, Andrea A. M. V. Matos, Denise D. C. S. Herrera, Miguel A. O. Yamamura, Anna M. Y. Freire, Marcos S. Gaspar, Luciane P. Caride, Elena |
| author_role |
author |
| author2 |
Silva, Andrea N. M. R. Souza, Marta Cristina O. Silva, Marlon V. Neves, Patrícia P. C. C. Silva, Andrea A. M. V. Matos, Denise D. C. S. Herrera, Miguel A. O. Yamamura, Anna M. Y. Freire, Marcos S. Gaspar, Luciane P. Caride, Elena |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pereira, Renata C. Silva, Andrea N. M. R. Souza, Marta Cristina O. Silva, Marlon V. Neves, Patrícia P. C. C. Silva, Andrea A. M. V. Matos, Denise D. C. S. Herrera, Miguel A. O. Yamamura, Anna M. Y. Freire, Marcos S. Gaspar, Luciane P. Caride, Elena |
| dc.subject.en.none.fl_str_mv |
Inactivated vaccine Protective response Yellow fever |
| topic |
Inactivated vaccine Protective response Yellow fever 03 Saúde e Bem-Estar |
| dc.subject.ods.none.fl_str_mv |
03 Saúde e Bem-Estar |
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Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil |
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2015 |
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2015 |
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2019-03-12T13:33:41Z |
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2019-03-12T13:33:41Z |
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PEREIRA, Renata C. et al. An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures. Vaccine, Amsterdam, v. 33, n. 35, p. 4261-4268, 2015. |
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10.1016/j.vaccine.2015.03.077 |
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PEREIRA, Renata C. et al. An inactivated yellow fever 17DD vaccine cultivated in Vero cell cultures. Vaccine, Amsterdam, v. 33, n. 35, p. 4261-4268, 2015. 1873-2518 10.1016/j.vaccine.2015.03.077 |
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