Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei

Bibliographic Details
Main Author: Patrick, Kristin L.
Publication Date: 2008
Other Authors: Luz, Paula Mendes, Ruan, Jia-Peng, Shi, Huafang, Ullu, Elisabetta, Tschudi, Christian
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1111/j.1365-2958.2007.06057.x
Download full: https://arca.fiocruz.br/handle/icict/30487
Summary: Yale University Medical School. Department of Epidemiology and Public Health. New Haven, CT, USA.
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spelling Patrick, Kristin L.Luz, Paula MendesRuan, Jia-PengShi, HuafangUllu, ElisabettaTschudi, Christian2018-12-10T15:36:44Z2018-12-10T15:36:44Z2008PATRICK, K. L. et al. Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei. Molecular microbiology, v. 67, n. 2, p. 435-447, 2008.0950-382Xhttps://arca.fiocruz.br/handle/icict/3048710.1111/j.1365-2958.2007.06057.xengWilleyGenomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma bruceiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleYale University Medical School. Department of Epidemiology and Public Health. New Haven, CT, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil./ Yale University Medical School. Department of Epidemiology and Public Health. New Haven, CT, USA.Yale University Medical School. Department of Epidemiology and Public Health. New Haven, CT, USA.Yale University Medical School. Department of Internal Medicine. New Haven, CT, USA.Yale University Medical School. Department of Internal Medicine. New Haven, CT, USA./ Yale University Medical School. Department of Cell Biology. New Haven, CT, USA.Yale University Medical School. Department of Epidemiology and Public Health. New Haven, CT, USA./ Yale University Medical School. Department of Internal Medicine. New Haven, CT, USA.The Trypanosoma brucei genome is colonized by the site-specific non-LTR retrotransposon SLACS, or spliced leader-associated conserved sequence, which integrates exclusively into the spliced leader (SL) RNA genes. Although there is evidence that the RNA interference (RNAi) machinery regulates SLACS transcript levels, we do not know whether RNAi deficiency affects the genomic stability of SLACS, nor do we understand the mechanism of SLACS transcription. Here, we report that prolonged culturing of RNAi-deficient T. brucei cells, but not wild-type cells, results in genomic rearrangements of SLACS. Furthermore, two populations of SLACS transcripts persist in RNAi-deficient cells: a full-length transcript of approximately 7 kb and a heterogeneous population of small SLACS transcripts ranging in size from 450 to 550 nt. We provide evidence that SLACS transcription initiates at the +1 of the interrupted SL RNA gene and proceeds into the 5' UTR and open reading frame 1 (ORF1). This transcription is carried out by an RNA polymerase with alpha-amanitin sensitivity reminiscent of SL RNA synthesis and is dependent on the SL RNA promoter. Additionally, we show that both sense and antisense small SLACS transcripts originate from ORF1 and that they are associated with proteins in vivo. We speculate that the small SLACS transcripts serve as substrates for the production of siRNAs to regulate SLACS expression.Gene RearrangementTrypanosoma bruceiRNA InterferenceRNA, Spliced LeaderRNA, ProtozoanRNA ProcessingRetroelementsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://arca.fiocruz.br/bitstreams/22d42861-c6d2-465a-953f-9280e56649da/download586c046dcfeef936e32f0323bb9a47c0MD51falseAnonymousREADORIGINALGenomic rearrangements_Paula_Luz_INI_Lapclin-AIDS_2008.pdfGenomic rearrangements_Paula_Luz_INI_Lapclin-AIDS_2008.pdfapplication/pdf554370https://arca.fiocruz.br/bitstreams/43ce7e06-7176-42ca-9672-a0577dc8dcbc/download209a78233c11fd3619874fe8e543b097MD52trueAnonymousREADTEXTGenomic rearrangements_Paula_Luz_INI_Lapclin-AIDS_2008.pdf.txtGenomic rearrangements_Paula_Luz_INI_Lapclin-AIDS_2008.pdf.txtExtracted texttext/plain65276https://arca.fiocruz.br/bitstreams/c78bc38d-3990-422e-a61d-dbccee27b593/downloadccacf15743f10a17ab5fbca01e384c16MD55falseAnonymousREADTHUMBNAILGenomic rearrangements_Paula_Luz_INI_Lapclin-AIDS_2008.pdf.jpgGenomic rearrangements_Paula_Luz_INI_Lapclin-AIDS_2008.pdf.jpgGenerated Thumbnailimage/jpeg5680https://arca.fiocruz.br/bitstreams/3613c069-805c-400f-8906-a8cb8d7f4cbd/downloadb08703061d556fe9182f4b53bf283131MD56falseAnonymousREADicict/304872025-08-03 15:46:30.806open.accessoai:arca.fiocruz.br:icict/30487https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-08-03T18:46:30Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
title Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
spellingShingle Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
Patrick, Kristin L.
Gene Rearrangement
Trypanosoma brucei
RNA Interference
RNA, Spliced Leader
RNA, Protozoan
RNA Processing
Retroelements
title_short Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
title_full Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
title_fullStr Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
title_full_unstemmed Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
title_sort Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
author Patrick, Kristin L.
author_facet Patrick, Kristin L.
Luz, Paula Mendes
Ruan, Jia-Peng
Shi, Huafang
Ullu, Elisabetta
Tschudi, Christian
author_role author
author2 Luz, Paula Mendes
Ruan, Jia-Peng
Shi, Huafang
Ullu, Elisabetta
Tschudi, Christian
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Patrick, Kristin L.
Luz, Paula Mendes
Ruan, Jia-Peng
Shi, Huafang
Ullu, Elisabetta
Tschudi, Christian
dc.subject.en.none.fl_str_mv Gene Rearrangement
Trypanosoma brucei
RNA Interference
RNA, Spliced Leader
RNA, Protozoan
RNA Processing
Retroelements
topic Gene Rearrangement
Trypanosoma brucei
RNA Interference
RNA, Spliced Leader
RNA, Protozoan
RNA Processing
Retroelements
description Yale University Medical School. Department of Epidemiology and Public Health. New Haven, CT, USA.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2018-12-10T15:36:44Z
dc.date.available.fl_str_mv 2018-12-10T15:36:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PATRICK, K. L. et al. Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei. Molecular microbiology, v. 67, n. 2, p. 435-447, 2008.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/30487
dc.identifier.issn.none.fl_str_mv 0950-382X
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2958.2007.06057.x
identifier_str_mv PATRICK, K. L. et al. Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei. Molecular microbiology, v. 67, n. 2, p. 435-447, 2008.
0950-382X
10.1111/j.1365-2958.2007.06057.x
url https://arca.fiocruz.br/handle/icict/30487
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Willey
publisher.none.fl_str_mv Willey
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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