A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete

Detalhes bibliográficos
Autor(a) principal: Wunder Junior, Elsio Augusto
Data de Publicação: 2016
Outros Autores: Figueira, Cláudio Pereira, Benaroudj, Nadia, Hu, Bo, Tong, Brian A., Trajtenberg, Felipe, Liu, Jun, Reis, Mitermayer Galvão dos, Charon, Nyles W., Buschiazzo, Alejandro, Picardeau, Mathieu, Ko, Albert Icksang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1111/mmi.13403
Texto Completo: https://arca.fiocruz.br/handle/icict/15272
Resumo: National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation.
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spelling Wunder Junior, Elsio AugustoFigueira, Cláudio PereiraBenaroudj, NadiaHu, BoTong, Brian A.Trajtenberg, FelipeLiu, JunReis, Mitermayer Galvão dosCharon, Nyles W.Buschiazzo, AlejandroPicardeau, MathieuKo, Albert Icksang2016-08-16T16:39:34Z2016-08-16T16:39:34Z2016WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016.0950-382Xhttps://arca.fiocruz.br/handle/icict/1527210.1111/mmi.13403National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation.Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur. Unit of Biology of Spirochetes. Paris, FranceUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USAUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USAInstitut Pasteur de Montevideo. Laboratory of Molecular & Structural Microbiology. Montevideo, UruguayUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilWest Virginia University. Department of Microbiology and ImmunologyMorgantown. WV, USAUniversity of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USA / Institute Pasteur. Department of Structural Biology and Chemistry. Paris, France.Institut Pasteur. Unit of Biology of Spirochetes. Paris, FranceYale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilLeptospira are unique among bacteria based on their helical cell morphology with hook-shaped ends and the presence of periplasmic flagella (PF) with pronounced spontaneous supercoiling. The factors that provoke such supercoiling, as well as the role that PF coiling plays in generating the characteristic hook-end cell morphology and motility, have not been elucidated. We have now identified an abundant protein from the pathogen L. interrogans, exposed on the PF surface, and named it Flagellar-coiling protein A (FcpA). The gene encoding FcpA is highly conserved among Leptospira and was not found in other bacteria. fcpA(-) mutants, obtained from clinical isolates or by allelic exchange, had relatively straight, smaller-diameter PF, and were not able to produce translational motility. These mutants lost their ability to cause disease in the standard hamster model of leptospirosis. Complementation of fcpA restored the wild-type morphology, motility and virulence phenotypes. In summary, we identified a novel Leptospira 36-kDa protein, the main component of the spirochete's PF sheath, and a key determinant of the flagella's coiled structure. FcpA is essential for bacterial translational motility and to enable the spirochete to penetrate the host, traverse tissue barriers, disseminate to cause systemic infection and reach target organs.engWilleyA novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirocheteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeptospiraLeptospirosisSpirocheteVirulenceFlagellaPhenotypesAnimalsCricetinaeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/8699eb43-43c1-4e07-afdf-8b010c54d716/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALWunder Jr EA A novel flagellar....pdfWunder Jr EA A novel flagellar....pdfapplication/pdf1300862https://arca.fiocruz.br/bitstreams/c0595ceb-c6dc-4d5b-b188-01fd6fd279c1/download33ea6cd0220c1627323cc95cb41f50cfMD52trueAnonymousREADTEXTWunder Jr EA A novel flagellar....pdf.txtWunder Jr EA A novel flagellar....pdf.txtExtracted texttext/plain71551https://arca.fiocruz.br/bitstreams/fcfdb0ee-4dcc-40c9-802f-dc744dd0c873/download87f3d486fc4b2385faf8fddea78270d7MD57falseAnonymousREADTHUMBNAILWunder Jr EA A novel flagellar....pdf.jpgWunder Jr EA A novel flagellar....pdf.jpgGenerated Thumbnailimage/jpeg6304https://arca.fiocruz.br/bitstreams/1c6220cc-6086-4b67-91bd-59140e0bf98c/downloadd9937b3c545099b7a41dc8450bdf927aMD58falseAnonymousREADicict/152722025-08-03 15:46:24.776open.accessoai:arca.fiocruz.br:icict/15272https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-08-03T18:46:24Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
title A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
spellingShingle A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
Wunder Junior, Elsio Augusto
Leptospira
Leptospirosis
Spirochete
Virulence
Flagella
Phenotypes
Animals
Cricetinae
title_short A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
title_full A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
title_fullStr A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
title_full_unstemmed A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
title_sort A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
author Wunder Junior, Elsio Augusto
author_facet Wunder Junior, Elsio Augusto
Figueira, Cláudio Pereira
Benaroudj, Nadia
Hu, Bo
Tong, Brian A.
Trajtenberg, Felipe
Liu, Jun
Reis, Mitermayer Galvão dos
Charon, Nyles W.
Buschiazzo, Alejandro
Picardeau, Mathieu
Ko, Albert Icksang
author_role author
author2 Figueira, Cláudio Pereira
Benaroudj, Nadia
Hu, Bo
Tong, Brian A.
Trajtenberg, Felipe
Liu, Jun
Reis, Mitermayer Galvão dos
Charon, Nyles W.
Buschiazzo, Alejandro
Picardeau, Mathieu
Ko, Albert Icksang
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wunder Junior, Elsio Augusto
Figueira, Cláudio Pereira
Benaroudj, Nadia
Hu, Bo
Tong, Brian A.
Trajtenberg, Felipe
Liu, Jun
Reis, Mitermayer Galvão dos
Charon, Nyles W.
Buschiazzo, Alejandro
Picardeau, Mathieu
Ko, Albert Icksang
dc.subject.en.none.fl_str_mv Leptospira
Leptospirosis
Spirochete
Virulence
Flagella
Phenotypes
Animals
Cricetinae
topic Leptospira
Leptospirosis
Spirochete
Virulence
Flagella
Phenotypes
Animals
Cricetinae
description National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-08-16T16:39:34Z
dc.date.available.fl_str_mv 2016-08-16T16:39:34Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/15272
dc.identifier.issn.none.fl_str_mv 0950-382X
dc.identifier.doi.none.fl_str_mv 10.1111/mmi.13403
identifier_str_mv WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016.
0950-382X
10.1111/mmi.13403
url https://arca.fiocruz.br/handle/icict/15272
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Willey
publisher.none.fl_str_mv Willey
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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