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BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge

Bibliographic Details
Main Author: Ramírez, Laura
Publication Date: 2010
Other Authors: Iborra, Salvador, Cortés, Jimena, Bonay, Pedro, Alonso, Carlos, Barral Netto, Manoel, Soto, Manuel
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://arca.fiocruz.br/handle/icict/7370
Summary: Universidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, Spain
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spelling Ramírez, LauraIborra, SalvadorCortés, JimenaBonay, PedroAlonso, CarlosBarral Netto, ManoelSoto, Manuel2014-02-27T18:59:46Z2014-02-27T18:59:46Z2010RAMÍREZ, L. et al. BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge. Journal of Biomedicine and Biotechnology, p. 1-10, 2010.1110-725110.1155/2010/181690https://arca.fiocruz.br/handle/icict/7370engHindawi Publishing CorporationBALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challengeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, SpainInstituto de Salud Carlos III. Centro Nacional de Microbiología. Unidad de Inmunología Viral. Majadahonda, Madrid, SpainUniversidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, SpainUniversidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, SpainUniversidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, SpainLeishmaniasis is an increasing public health problem and effective vaccines are not currently available. We have previously demonstrated that vaccination with ribosomal proteins extracts administered in combination of CpG oligodeoxynucleotides protects susceptible BALB/c mice against primary Leishmania major infection. Here, we evaluate the long-term immunity to secondary infection conferred by this vaccine.We show that vaccinated and infected BALB/c mice were able to control a secondary Leishmania major challenge, since no inflammation and very low number of parasites were observed in the site of reinfection. In addition, although an increment in the parasite burden was observed in the draining lymph nodes of the primary site of infection we did not detected inflammatory lesions at that site. Resistance against reinfection correlated to a predominant Th1 response against parasite antigens. Thus, cell cultures established from spleens and the draining lymph node of the secondary site of infection produced high levels of parasite specific IFN-γ in the absence of IL-4 and IL-10 cytokine production. In addition, reinfected mice showed a high IgG2a/IgG1 ratio for anti-Leishmania antibodies. Our results suggest that ribosomal vaccine, which prevents pathology in a primary challenge, in combination with parasite persistence might be effective for long term maintenance of immunity.Leishmania major/imunologiaVacinas contra Leishmaniose/imunologiaLeishmaniose Cutânea/imunologiaOligodesoxirribonucleotídeos/imunologiaProteínas de Protozoários/imunologiaProteínas Ribossômicas/imunologiaAnimaisAntígenos de Protozoários/imunologiaCitocinas/imunologiaCitocinas/metabolismoModelos Animais de DoençasFemininoVacinas contra Leishmaniose/farmacologiaLeishmaniose Cutânea/prevenção & controleCamundongosCamundongos Endogâmicos BALB Cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRamírez L BALB-C mice....pdfRamírez L BALB-C mice....pdfapplication/pdf908126https://arca.fiocruz.br/bitstreams/47f7d369-ba00-4e68-a5dc-cc606d231ef9/download5797630a6ef48b0c7b8f8c5b7b97621fMD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://arca.fiocruz.br/bitstreams/3f1b9776-7a6a-4f02-b0d6-001390990d70/download7d48279ffeed55da8dfe2f8e81f3b81fMD52falseAnonymousREADTEXTRamírez L BALB-C mice....pdf.txtRamírez L BALB-C mice....pdf.txtExtracted texttext/plain40990https://arca.fiocruz.br/bitstreams/b1022df7-1c1d-47f4-8937-4f301992fee3/downloadb475f8d442373e5d3986ee52ec29ee23MD58falseAnonymousREADTHUMBNAILRamírez L BALB-C mice....pdf.jpgRamírez L BALB-C mice....pdf.jpgGenerated Thumbnailimage/jpeg5303https://arca.fiocruz.br/bitstreams/aeb86244-08cf-46f0-9cce-086e0f57b3ac/download41986109dc3b9c63c83beae726d4e5b9MD59falseAnonymousREADicict/73702025-07-29 19:53:46.492open.accessoai:arca.fiocruz.br:icict/7370https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T22:53:46Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
title BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
spellingShingle BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
Ramírez, Laura
Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/imunologia
Oligodesoxirribonucleotídeos/imunologia
Proteínas de Protozoários/imunologia
Proteínas Ribossômicas/imunologia
Animais
Antígenos de Protozoários/imunologia
Citocinas/imunologia
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Vacinas contra Leishmaniose/farmacologia
Leishmaniose Cutânea/prevenção & controle
Camundongos
Camundongos Endogâmicos BALB C
title_short BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
title_full BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
title_fullStr BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
title_full_unstemmed BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
title_sort BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge
author Ramírez, Laura
author_facet Ramírez, Laura
Iborra, Salvador
Cortés, Jimena
Bonay, Pedro
Alonso, Carlos
Barral Netto, Manoel
Soto, Manuel
author_role author
author2 Iborra, Salvador
Cortés, Jimena
Bonay, Pedro
Alonso, Carlos
Barral Netto, Manoel
Soto, Manuel
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramírez, Laura
Iborra, Salvador
Cortés, Jimena
Bonay, Pedro
Alonso, Carlos
Barral Netto, Manoel
Soto, Manuel
dc.subject.decs.none.fl_str_mv Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/imunologia
Oligodesoxirribonucleotídeos/imunologia
Proteínas de Protozoários/imunologia
Proteínas Ribossômicas/imunologia
Animais
Antígenos de Protozoários/imunologia
Citocinas/imunologia
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Vacinas contra Leishmaniose/farmacologia
Leishmaniose Cutânea/prevenção & controle
Camundongos
Camundongos Endogâmicos BALB C
topic Leishmania major/imunologia
Vacinas contra Leishmaniose/imunologia
Leishmaniose Cutânea/imunologia
Oligodesoxirribonucleotídeos/imunologia
Proteínas de Protozoários/imunologia
Proteínas Ribossômicas/imunologia
Animais
Antígenos de Protozoários/imunologia
Citocinas/imunologia
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Vacinas contra Leishmaniose/farmacologia
Leishmaniose Cutânea/prevenção & controle
Camundongos
Camundongos Endogâmicos BALB C
description Universidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, Spain
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2014-02-27T18:59:46Z
dc.date.available.fl_str_mv 2014-02-27T18:59:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMÍREZ, L. et al. BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge. Journal of Biomedicine and Biotechnology, p. 1-10, 2010.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/7370
dc.identifier.issn.none.fl_str_mv 1110-7251
10.1155/2010/181690
identifier_str_mv RAMÍREZ, L. et al. BALB/c mice vaccinated with Leishmania major ribosomal proteins extracts combined with CpG oligodeoxynucleotides become resistant to disease caused by a secondary parasite challenge. Journal of Biomedicine and Biotechnology, p. 1-10, 2010.
1110-7251
10.1155/2010/181690
url https://arca.fiocruz.br/handle/icict/7370
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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