Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity

Bibliographic Details
Main Author: Barril, P. A.
Publication Date: 2015
Other Authors: Fumian, T. M., Prez, V. E., Gil, P. I., Martinez, L. C., Giordano, M. O., Masachessi, G., Isa, M. B., Ferreyra, L. J., Ré, V. E., Miagostovich, M., Pavan, J. V., Nates, S. V.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/j.envres.2015.03.004
Download full: https://arca.fiocruz.br/handle/icict/12106
Summary: 2016-03-31
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spelling Barril, P. A.Fumian, T. M.Prez, V. E.Gil, P. I.Martinez, L. C.Giordano, M. O.Masachessi, G.Isa, M. B.Ferreyra, L. J.Ré, V. E.Miagostovich, M.Pavan, J. V.Nates, S. V.2015-11-03T13:48:00Z2016-04-01T07:30:09Z2015BARRIL, P. A. et al. Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity. Environmental Research, v.138, p.409-415, Apr. 2015.0013-9351https://arca.fiocruz.br/handle/icict/1210610.1016/j.envres.2015.03.0041096-0953engScienceDirectRotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-03-31Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”. Córdoba, Argentina.In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from Córdoba city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. RT-nested PCR was applied for rotavirus detection, and VP7/VP4 characterization and real-time PCR for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time PCR in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than 18 °C and also higher, although not statistically different during drier weather. VP7 and VP4 gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.RotavirusSeasonalitySewageEnvironmental monitoringMeteorological variablesArgentinaRotavirusEsgotosMonitoramento AmbientalÁrea UrbanaArgentina06 Água potável e saneamentoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://arca.fiocruz.br/bitstreams/1b7c8c65-65e7-4e14-a8ce-e8f6ac74fb94/download8b4c200b4e10021c5683c6ccaba07169MD51falseAnonymousREADORIGINALtm_fumian_etal_IOC_2015.pdftm_fumian_etal_IOC_2015.pdfapplication/pdf661923https://arca.fiocruz.br/bitstreams/b8f914fa-656a-4570-b8cb-5e39fdf1d69c/downloadb01671b4af5f7ae00971df9815e4f27bMD52trueAnonymousREAD2016-03-31TEXTtm_fumian_etal_IOC_2015.pdf.txttm_fumian_etal_IOC_2015.pdf.txtExtracted texttext/plain51092https://arca.fiocruz.br/bitstreams/d519f950-20ca-490e-ad48-655880a0226a/download709d5c1fbee1904c02acf87b5f7f8d8eMD57falseAnonymousREAD2016-03-31THUMBNAILtm_fumian_etal_IOC_2015.pdf.jpgtm_fumian_etal_IOC_2015.pdf.jpgGenerated Thumbnailimage/jpeg5816https://arca.fiocruz.br/bitstreams/3ecd3aa7-18ca-4fb1-8ff4-6888b0332e77/download496ba203a6260e7584ab242501084d38MD58falseAnonymousREAD2016-03-31icict/121062025-07-29 19:34:34.705open.accessoai:arca.fiocruz.br:icict/12106https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T22:34:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
spellingShingle Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
Barril, P. A.
Rotavirus
Seasonality
Sewage
Environmental monitoring
Meteorological variables
Argentina
Rotavirus
Esgotos
Monitoramento Ambiental
Área Urbana
Argentina
06 Água potável e saneamento
title_short Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_full Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_fullStr Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_full_unstemmed Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_sort Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
author Barril, P. A.
author_facet Barril, P. A.
Fumian, T. M.
Prez, V. E.
Gil, P. I.
Martinez, L. C.
Giordano, M. O.
Masachessi, G.
Isa, M. B.
Ferreyra, L. J.
Ré, V. E.
Miagostovich, M.
Pavan, J. V.
Nates, S. V.
author_role author
author2 Fumian, T. M.
Prez, V. E.
Gil, P. I.
Martinez, L. C.
Giordano, M. O.
Masachessi, G.
Isa, M. B.
Ferreyra, L. J.
Ré, V. E.
Miagostovich, M.
Pavan, J. V.
Nates, S. V.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barril, P. A.
Fumian, T. M.
Prez, V. E.
Gil, P. I.
Martinez, L. C.
Giordano, M. O.
Masachessi, G.
Isa, M. B.
Ferreyra, L. J.
Ré, V. E.
Miagostovich, M.
Pavan, J. V.
Nates, S. V.
dc.subject.en.none.fl_str_mv Rotavirus
Seasonality
Sewage
Environmental monitoring
Meteorological variables
Argentina
topic Rotavirus
Seasonality
Sewage
Environmental monitoring
Meteorological variables
Argentina
Rotavirus
Esgotos
Monitoramento Ambiental
Área Urbana
Argentina
06 Água potável e saneamento
dc.subject.decs.none.fl_str_mv Rotavirus
Esgotos
Monitoramento Ambiental
Área Urbana
Argentina
dc.subject.ods.none.fl_str_mv 06 Água potável e saneamento
description 2016-03-31
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-11-03T13:48:00Z
dc.date.issued.fl_str_mv 2015
dc.date.available.fl_str_mv 2016-04-01T07:30:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARRIL, P. A. et al. Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity. Environmental Research, v.138, p.409-415, Apr. 2015.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/12106
dc.identifier.issn.none.fl_str_mv 0013-9351
dc.identifier.doi.none.fl_str_mv 10.1016/j.envres.2015.03.004
dc.identifier.eissn.none.fl_str_mv 1096-0953
identifier_str_mv BARRIL, P. A. et al. Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity. Environmental Research, v.138, p.409-415, Apr. 2015.
0013-9351
10.1016/j.envres.2015.03.004
1096-0953
url https://arca.fiocruz.br/handle/icict/12106
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ScienceDirect
publisher.none.fl_str_mv ScienceDirect
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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