Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy
| Main Author: | |
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| Publication Date: | 2021 |
| Other Authors: | , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1155/2021/8815206 |
| Download full: | https://arca.fiocruz.br/handle/icict/47656 |
Summary: | Print Fiocruz-CAPES Program |
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Oliveira, Anna Lethicia L.Santos, Gisele G. L.Espirito-Santo, Renan F.Silva, Gessica Sabrina A.Evangelista, Afrânio F.Silva, Daniela N.Soares, Milena Botelho PereiraVillarreal, Cristiane Flora2021-06-12T12:29:11Z2021-06-12T12:29:11Z2021OLIVEIRA, Anna Lethicia L. et al. Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy. Stem Cells International, v. 2021, n. 8815206, p. 1-14, 7 Jan. 2021.1687-966Xhttps://arca.fiocruz.br/handle/icict/4765610.1155/2021/88152061687-9678Print Fiocruz-CAPES ProgramFundação de Amparo à Pesquisa do Estado da Bahia (FAPESB; grant number RED0010/2012)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil (Finance Code 001)Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Instituto SENAI de Sistemas Avançados de Saúde. Salvador, BA, Brasil / Serviço Nacional de Aprendizagem Industrial. Centro Integrado de Manufatura e Tecnologia. Centro Universitário. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto SENAI de Sistemas Avançados de Saúde. Salvador, BA, Brasil / Serviço Nacional de Aprendizagem Industrial. Centro Integrado de Manufatura e Tecnologia. Centro Universitário. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Painful neuropathy is a common adverse effect of oxaliplatin (OXL), a platinum-derivative chemotherapeutic agent. Oxidative stress and mitochondrial dysfunction are key factors contributing to the development of OXL-induced peripheral neuropathy (OIPN). Based on the antioxidant and antinociceptive properties of mesenchymal stem/stromal cells (MSC), the present study tested the hypothesis that MSC induce antinociceptive effects during OIPN by promoting regulation of redox environment and mitochondrial homeostasis in the nociceptive primary afferents. C57Bl/6 mice submitted to the OXL-chronic neuropathy induction protocol by repeated intravenous administration of OXL (1 mg/kg) were evaluated to determine the paw mechanical and thermal nociceptive thresholds using the von Frey filaments and cold plate tests, respectively. Two weeks after the neuropathy induction, mice were treated with bone marrow-derived MSC (1 × 106), vehicle, or gabapentin (GBP, 70 mg/kg). Four weeks later, mitochondrial morphology, gene expression profile, and oxidative stress markers in the sciatic nerve and dorsal root ganglia (DRG) were evaluated by transmission electron microscopy, RT-qPCR, and biochemical assays, respectively. OXL-treated mice presented behavioral signs of sensory neuropathy, such as mechanical allodynia and thermal hyperalgesia. The behavioral painful neuropathy was completely reverted by a single administration of MSC, while the daily treatment with GBP induced only a short-lived antinociceptive effect. The ultrastructural analysis of the sciatic nerve and DRG of OIPN mice revealed a high proportion of atypical mitochondria in both myelinated and unmyelinated fibers. Importantly, this mitochondrial atypia was strongly reduced in MSC-treated neuropathic mice. Moreover, MSC-treated neuropathic mice showed upregulation of Sod and Nrf2 mRNA in the sciatic nerve and DRG. In line with this result, MSC reduced markers of nitrosative stress and lipid peroxidation in the sciatic nerve and DRG from OIPN mice. Our data suggest that the reestablishment of redox homeostasis in the nociceptive primary afferents is a mechanism by which MSC transplantation reverts the OXL-induced chronic painful neuropathy.engHindawiHomeostaseDoenças do sistema nervoso periféricoMicroscopia eletrônica de transmissãoMedula ósseaPeroxidação de lipídeosHomeostasisPeripheral nervous system diseasesTransmission electron microscopyBone marrowLipid peroxidation03 Saúde e Bem-EstarReestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://arca.fiocruz.br/bitstreams/ec8e151e-513b-4a04-ac75-8bc87f507074/download36b51ef91c52b5338d9d29ba0cc807bcMD51falseAnonymousREADORIGINALOliveira, Anna Lethicia L Reestablishment....pdfOliveira, Anna Lethicia L Reestablishment....pdfapplication/pdf3846792https://arca.fiocruz.br/bitstreams/66ce9c31-e95e-4b03-9577-bab58f588bcb/download59b00c8dcce862e870001a923c125b98MD52trueAnonymousREADTEXTOliveira, Anna Lethicia L Reestablishment....pdf.txtOliveira, Anna Lethicia L Reestablishment....pdf.txtExtracted texttext/plain69176https://arca.fiocruz.br/bitstreams/ecf36053-29c3-4f5b-8c87-b68e348b2aba/download0c1333fa12a432143d2c3bebd7a03db4MD55falseAnonymousREADTHUMBNAILOliveira, Anna Lethicia L Reestablishment....pdf.jpgOliveira, Anna Lethicia L Reestablishment....pdf.jpgGenerated Thumbnailimage/jpeg5306https://arca.fiocruz.br/bitstreams/844b021d-c8b7-4675-ad9e-ace6ee29da38/download75193eb2dc4b7cc2b76822f3b8ebbab7MD56falseAnonymousREADicict/476562025-07-30 02:02:42.18open.accessoai:arca.fiocruz.br:icict/47656https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T05:02:42Repositório Institucional da FIOCRUZ (ARCA) - 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Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| title |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| spellingShingle |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy Oliveira, Anna Lethicia L. Homeostase Doenças do sistema nervoso periférico Microscopia eletrônica de transmissão Medula óssea Peroxidação de lipídeos Homeostasis Peripheral nervous system diseases Transmission electron microscopy Bone marrow Lipid peroxidation 03 Saúde e Bem-Estar |
| title_short |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| title_full |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| title_fullStr |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| title_full_unstemmed |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| title_sort |
Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy |
| author |
Oliveira, Anna Lethicia L. |
| author_facet |
Oliveira, Anna Lethicia L. Santos, Gisele G. L. Espirito-Santo, Renan F. Silva, Gessica Sabrina A. Evangelista, Afrânio F. Silva, Daniela N. Soares, Milena Botelho Pereira Villarreal, Cristiane Flora |
| author_role |
author |
| author2 |
Santos, Gisele G. L. Espirito-Santo, Renan F. Silva, Gessica Sabrina A. Evangelista, Afrânio F. Silva, Daniela N. Soares, Milena Botelho Pereira Villarreal, Cristiane Flora |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Anna Lethicia L. Santos, Gisele G. L. Espirito-Santo, Renan F. Silva, Gessica Sabrina A. Evangelista, Afrânio F. Silva, Daniela N. Soares, Milena Botelho Pereira Villarreal, Cristiane Flora |
| dc.subject.other.none.fl_str_mv |
Homeostase Doenças do sistema nervoso periférico Microscopia eletrônica de transmissão Medula óssea Peroxidação de lipídeos |
| topic |
Homeostase Doenças do sistema nervoso periférico Microscopia eletrônica de transmissão Medula óssea Peroxidação de lipídeos Homeostasis Peripheral nervous system diseases Transmission electron microscopy Bone marrow Lipid peroxidation 03 Saúde e Bem-Estar |
| dc.subject.en.none.fl_str_mv |
Homeostasis Peripheral nervous system diseases Transmission electron microscopy Bone marrow Lipid peroxidation |
| dc.subject.ods.none.fl_str_mv |
03 Saúde e Bem-Estar |
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Print Fiocruz-CAPES Program |
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2021 |
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2021-06-12T12:29:11Z |
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2021-06-12T12:29:11Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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OLIVEIRA, Anna Lethicia L. et al. Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy. Stem Cells International, v. 2021, n. 8815206, p. 1-14, 7 Jan. 2021. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/47656 |
| dc.identifier.issn.none.fl_str_mv |
1687-966X |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2021/8815206 |
| dc.identifier.eissn.none.fl_str_mv |
1687-9678 |
| identifier_str_mv |
OLIVEIRA, Anna Lethicia L. et al. Reestablishment of Redox homeostasis in the nociceptive primary afferent as a mechanism of antinociception promoted by mesenchymal stem/stromal cells in oxaliplatin-Induced chronic peripheral neuropathy. Stem Cells International, v. 2021, n. 8815206, p. 1-14, 7 Jan. 2021. 1687-966X 10.1155/2021/8815206 1687-9678 |
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Hindawi |
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Hindawi |
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