Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]

Odero, Joel; Gomes, Bruno; Fillinger, Ulrike

Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]

Bibliographic Details
Main Author:	Odero, Joel
Publication Date:	2018
Other Authors:	Gomes, Bruno, Fillinger, Ulrike
Format:	Article
Language:	eng
Source:	Repositório Institucional da FIOCRUZ (ARCA)
DOI:	10.12688/wellcomeopenres.14193.1
Download full:	https://arca.fiocruz.br/handle/icict/31790
Summary:	Malaria Programme. International Centre of Insect Physiology and Ecology. Thomas Odhiambo Campus. Mbita, Kenya.

Item metadata

id	CRUZ_8ce38d517ba2761750d23d0e95ffc65b
oai_identifier_str	oai:arca.fiocruz.br:icict/31790
network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Odero, JoelGomes, BrunoFillinger, Ulrike2019-02-20T16:07:59Z2019-02-20T16:07:59Z2018ODERO, Joel; GOMES, Bruno; FILLINGER, Ulrike. Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]. Welcome Open Research, p. 1-7, 6 June 2018.2398-502Xhttps://arca.fiocruz.br/handle/icict/3179010.12688/wellcomeopenres.14193.1engF1000ResearchHabitats aquáticosDNA ambientalLarvasMonitoramentoAnophelesEnvironmental DNA (eDNA)An. gambiae s.s.An. arabiensisLarvaeAquatic habitatsMonitoringDetection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalaria Programme. International Centre of Insect Physiology and Ecology. Thomas Odhiambo Campus. Mbita, Kenya.Department of Vector Biology. Liverpool School of Tropical Medicine. Liverpool, UK / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Malaria Programme. International Centre of Insect Physiology and Ecology. Thomas Odhiambo Campus. Mbita, Kenya.Liverpool School of Tropical Medicine. Department of Vector Biology. Liverpool, UK.Background: Growing insecticide resistance and changes in biting and resting behavior of malaria vectors threaten efficacy of insecticide treated nets and indoor residual spraying. Larval source management (LSM) is a promising approach that can target mosquitoes irrespective of their behavior as adults. However, the use of traditional monitoring methods for immature stages of Anopheles mosquitoes is a major challenge to LSM due to the variability in their breeding habitats. We evaluate the use of an environmental DNA (eDNA) analysis technique in monitoring Anopheles gambiae sensu lato larvae in experimental aquatic habitats. Methods: eDNA was simultaneously sampled and extracted from different volumes of water, number of larvae, and occupation time. Larval presence was detected using PCR and eDNA concentration in samples from 1 L habitats quantified using an IGS and cyt b TaqMan assays. The limit of detection of the two assays was tested and larval density correlated with eDNA positivity. Results: 74% of replicates in the 50 mL habitats were PCR positive with at least 6h required to get a signal from a single larva (0.02 larvae/mL). All 12 replicates where 1 L of water was used were positive with stronger PCR bands than replicates with the same larval density in 50 mL for 24 h. There was a correlation between larval densities and eDNA detection in both assays: IGS, r = 0.503, p = 0.047; and cyt b,r = 0.558, p = 0.025. There was stochasticity in eDNA detection rates, using both PCR and qPCR across all the dilutions. Conclusion: This study has demonstrated the potential use of eDNA analysis for detection and quantification of An. gambiae s.s. mosquito larvae in aquatic habitats. The stochasticity observed in eDNA detection suggest that this technique is best for monitoring aquatic habitats with many larvae at low densities.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/3c31eaf9-91f9-4ffb-ae96-8ad98d6de524/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALbruno_gomes_etal_IOC_2018.pdfbruno_gomes_etal_IOC_2018.pdfapplication/pdf1016514https://arca.fiocruz.br/bitstreams/d72840a9-0474-4eb8-a2e8-8ce00008ed2b/downloadd52bba878406ad1d3189091f588b789bMD52trueAnonymousREADTEXTbruno_gomes_etal_IOC_2018.pdf.txtbruno_gomes_etal_IOC_2018.pdf.txtExtracted texttext/plain65066https://arca.fiocruz.br/bitstreams/c7d9e6c9-19ed-4946-8fdc-0ae3b827c5e4/download0753290baa0b2ba193d2aa48f0cd2d8dMD55falseAnonymousREADTHUMBNAILbruno_gomes_etal_IOC_2018.pdf.jpgbruno_gomes_etal_IOC_2018.pdf.jpgGenerated Thumbnailimage/jpeg5952https://arca.fiocruz.br/bitstreams/a5a985a0-7200-4863-921d-c894e41f0187/download374c8ecf54262e87730ce6d492b8bbd4MD56falseAnonymousREADicict/317902025-07-30 01:30:19.495open.accessoai:arca.fiocruz.br:icict/31790https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T04:30:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)falseQ0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4K
dc.title.none.fl_str_mv	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
title	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
spellingShingle	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations] Odero, Joel Habitats aquáticos DNA ambiental Larvas Monitoramento Anopheles Environmental DNA (eDNA) An. gambiae s.s. An. arabiensis Larvae Aquatic habitats Monitoring
title_short	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
title_full	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
title_fullStr	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
title_full_unstemmed	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
title_sort	Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]
author	Odero, Joel
author_facet	Odero, Joel Gomes, Bruno Fillinger, Ulrike
author_role	author
author2	Gomes, Bruno Fillinger, Ulrike
author2_role	author author
dc.contributor.author.fl_str_mv	Odero, Joel Gomes, Bruno Fillinger, Ulrike
dc.subject.other.none.fl_str_mv	Habitats aquáticos DNA ambiental Larvas Monitoramento Anopheles
topic	Habitats aquáticos DNA ambiental Larvas Monitoramento Anopheles Environmental DNA (eDNA) An. gambiae s.s. An. arabiensis Larvae Aquatic habitats Monitoring
dc.subject.en.none.fl_str_mv	Environmental DNA (eDNA) An. gambiae s.s. An. arabiensis Larvae Aquatic habitats Monitoring
description	Malaria Programme. International Centre of Insect Physiology and Ecology. Thomas Odhiambo Campus. Mbita, Kenya.
publishDate	2018
dc.date.issued.fl_str_mv	2018
dc.date.accessioned.fl_str_mv	2019-02-20T16:07:59Z
dc.date.available.fl_str_mv	2019-02-20T16:07:59Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	ODERO, Joel; GOMES, Bruno; FILLINGER, Ulrike. Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]. Welcome Open Research, p. 1-7, 6 June 2018.
dc.identifier.uri.fl_str_mv	https://arca.fiocruz.br/handle/icict/31790
dc.identifier.issn.none.fl_str_mv	2398-502X
dc.identifier.doi.none.fl_str_mv	10.12688/wellcomeopenres.14193.1
identifier_str_mv	ODERO, Joel; GOMES, Bruno; FILLINGER, Ulrike. Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]. Welcome Open Research, p. 1-7, 6 June 2018. 2398-502X 10.12688/wellcomeopenres.14193.1
url	https://arca.fiocruz.br/handle/icict/31790
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	F1000Research
publisher.none.fl_str_mv	F1000Research
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://arca.fiocruz.br/bitstreams/3c31eaf9-91f9-4ffb-ae96-8ad98d6de524/download https://arca.fiocruz.br/bitstreams/d72840a9-0474-4eb8-a2e8-8ce00008ed2b/download https://arca.fiocruz.br/bitstreams/c7d9e6c9-19ed-4946-8fdc-0ae3b827c5e4/download https://arca.fiocruz.br/bitstreams/a5a985a0-7200-4863-921d-c894e41f0187/download
bitstream.checksum.fl_str_mv	5a560609d32a3863062d77ff32785d58 d52bba878406ad1d3189091f588b789b 0753290baa0b2ba193d2aa48f0cd2d8d 374c8ecf54262e87730ce6d492b8bbd4
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
_version_	1839716376793579520

Detection and quantification of Anopheles gambiae sensu lato mosquito larvae in experimental aquatic habitats using environmental DNA (eDNA). [version 1; referees: 2 approved, 2 approved with reservations]

Similar Items