Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania

Bibliographic Details
Main Author: Mendonça, Sergio Coutinho Furtado de
Publication Date: 2015
Other Authors: Cysne-Finkelstein, Lea, Matos, Denise Cristina de Souza
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3389/fimmu.2015.00524
Download full: https://arca.fiocruz.br/handle/icict/13771
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Mendonça, Sergio Coutinho Furtado deCysne-Finkelstein, LeaMatos, Denise Cristina de Souza2016-04-14T15:10:57Z2016-04-14T15:10:57Z2015MENDONÇA, Sergio Coutinho Furtado de; CYSNE-FINKELSTEIN, Léa; MATOS, Denise Cristina de Souza. Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania. Frontiers in Immunology, v.6, Article 524, 6p, Oct. 2015.1664-3224https://arca.fiocruz.br/handle/icict/1377110.3389/fimmu.2015.00524engFrontiers MediaKinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmaniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil.Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. In Leishmania amazonensis, KMP-11 is expressed in promastigotes and amastigotes. In both stages, the protein was found in association with membrane structures at the cell surface, flagellar pocket, and intracellular vesicles. More importantly, its surface expression is higher in amastigotes than in promastigotes and increases during metacyclogenesis. The increased expression of KMP-11 in metacyclic promastigotes, and especially in amastigotes, indicates a role for this molecule in the parasite relationship with the mammalian host. In this connection, we have shown that addition of KMP-11 exacerbates L. amazonensis infection in peritoneal macrophages from BALB/c mice by increasing interleukin (IL)-10 secretion and arginase activity while reducing nitric oxide production. The doses of KMP-11, the IL-10 levels, and the intracellular amastigote loads were strongly, positively, and significantly correlated. The increase in parasite load induced by KMP-11 was inhibited by anti-KMP-11 or anti-IL-10-neutralizing antibodies, but not by isotype controls. The neutralizing antibodies, but not the isotype controls, were also able to significantly decrease the parasite load in macrophages cultured without the addition of KMP-11, demonstrating that KMP-11-induced exacerbation of the infection is not dependent on the addition of exogenous KMP-11 and that the protein naturally expressed by the parasite is able to promote it. All these data indicate that KMP-11 acts as a virulence factor in L. amazonensis infection.LeishmaniaLeishmaniasisKMP-11Virulence factorVaccineLeishmaniaLeishmanioseVacinaFator de virulênciaLeishmania braziliensis03 Saúde e Bem-Estarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/a048b0e6-9531-4f0a-930e-0e1c2fb65a8d/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALsergio2_mendonça_etal_IOC_2015.pdfsergio2_mendonça_etal_IOC_2015.pdfapplication/pdf164599https://arca.fiocruz.br/bitstreams/47cf5728-20bd-407d-b97c-f3b3007da834/download2fb52f9528782857eccf99e933467e50MD52trueAnonymousREADTEXTsergio2_mendonça_etal_IOC_2015.pdf.txtsergio2_mendonça_etal_IOC_2015.pdf.txtExtracted texttext/plain46395https://arca.fiocruz.br/bitstreams/d983f52e-1fdd-435d-bed6-f6e962042f36/download0f97470e2cc91f0865e674966910bbf5MD57falseAnonymousREADTHUMBNAILsergio2_mendonça_etal_IOC_2015.pdf.jpgsergio2_mendonça_etal_IOC_2015.pdf.jpgGenerated Thumbnailimage/jpeg5533https://arca.fiocruz.br/bitstreams/dbe32d5d-fae7-4cb2-a184-bbe0f678c9de/download3579a240c6a44badc1f29c42bdcecf2aMD58falseAnonymousREADicict/137712025-07-29 18:48:40.913open.accessoai:arca.fiocruz.br:icict/13771https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T21:48:40Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
title Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
spellingShingle Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
Mendonça, Sergio Coutinho Furtado de
Leishmania
Leishmaniasis
KMP-11
Virulence factor
Vaccine
Leishmania
Leishmaniose
Vacina
Fator de virulência
Leishmania braziliensis
03 Saúde e Bem-Estar
title_short Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
title_full Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
title_fullStr Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
title_full_unstemmed Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
title_sort Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania
author Mendonça, Sergio Coutinho Furtado de
author_facet Mendonça, Sergio Coutinho Furtado de
Cysne-Finkelstein, Lea
Matos, Denise Cristina de Souza
author_role author
author2 Cysne-Finkelstein, Lea
Matos, Denise Cristina de Souza
author2_role author
author
dc.contributor.author.fl_str_mv Mendonça, Sergio Coutinho Furtado de
Cysne-Finkelstein, Lea
Matos, Denise Cristina de Souza
dc.subject.en.none.fl_str_mv Leishmania
Leishmaniasis
KMP-11
Virulence factor
Vaccine
topic Leishmania
Leishmaniasis
KMP-11
Virulence factor
Vaccine
Leishmania
Leishmaniose
Vacina
Fator de virulência
Leishmania braziliensis
03 Saúde e Bem-Estar
dc.subject.decs.none.fl_str_mv Leishmania
Leishmaniose
Vacina
Fator de virulência
Leishmania braziliensis
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-14T15:10:57Z
dc.date.available.fl_str_mv 2016-04-14T15:10:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENDONÇA, Sergio Coutinho Furtado de; CYSNE-FINKELSTEIN, Léa; MATOS, Denise Cristina de Souza. Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania. Frontiers in Immunology, v.6, Article 524, 6p, Oct. 2015.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/13771
dc.identifier.issn.none.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2015.00524
identifier_str_mv MENDONÇA, Sergio Coutinho Furtado de; CYSNE-FINKELSTEIN, Léa; MATOS, Denise Cristina de Souza. Kinetoplastid membrane protein-11 as a vaccine candidate and a virulence factor in Leishmania. Frontiers in Immunology, v.6, Article 524, 6p, Oct. 2015.
1664-3224
10.3389/fimmu.2015.00524
url https://arca.fiocruz.br/handle/icict/13771
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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