Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus

Bibliographic Details
Main Author: Cunha, J. M. Albuquerque
Publication Date: 2004
Other Authors: Mello, C. B., Garcia, Eloi S., Azambuja, P., Souza, W. de, Gonzalez, M. S., Nogueira, N. F. S.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02762004000800006
Download full: https://arca.fiocruz.br/handle/icict/44544
Summary: Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil.
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spelling Cunha, J. M. AlbuquerqueMello, C. B.Garcia, Eloi S.Azambuja, P.Souza, W. deGonzalez, M. S.Nogueira, N. F. S.2020-11-19T17:59:22Z2020-11-19T17:59:22Z2004CUNHA, J. M. Albuquerque et al. Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 9, p. 815-822, Dec. 2004.0074-0276https://arca.fiocruz.br/handle/icict/4454410.1590/S0074-027620040008000061678-8060engFiocruz/IOCMicrovilosidadesTriatomíneosMembranas perimicrovilaresHormôniosTriatomineMidgutPerimicrovillar membranesHormoneMicrovilliEffect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultra-estrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil.Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil.The effects of blood components, nerve-cord severance, and ecdysone therapy on the posterior midgut epithelial cells of 5th-instar Rhodnius prolixus nymphs 10 days after feeding were analyzed by transmission electron microscopy. Cutting the nerve-cord of the blood-fed insects partially reduced the development of microvilli and perimicrovillar membranes (PMM), and produced large vacuoles and small electrondense granules; insects fed on Ringer's saline diet exhibited well developed microvilli and low PMM production; swolled rough endoplasmatic reticulum and electrondense granules; Ringer's saline meal with ecdysone led to PMM development, glycogen particles, and several mitochondria in the cytoplasm; epithelial cells of the insects fed on Ringer's saline meal whose nerve-cord was severed showed heterogeneously distributed microvilli with reduced PMM production and a great quantity of mitochondria and glycogen in the cytoplasm; well developed microvilli and PMM were observed in nerve-cord severed insects fed on Ringer's saline meal with ecdysone; Ringer's saline diet containing hemoglobin recovered the release of PMM; and insects fed on human plasma showed slightly reduced PMM production, although the addition of ecdysone in the plasma led to a normal midgut ultrastructural organization. We suggest that the full development of microvilli and PMM in the epithelial cells depends on the abdominal distension in addition to ingestion of hemoglobin, and the release of ecdysone.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/b209def8-3d18-41ad-b74f-cc372e7663e6/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALEloiS_Garcia_etal_IOC_2004.pdfEloiS_Garcia_etal_IOC_2004.pdfapplication/pdf2043992https://arca.fiocruz.br/bitstreams/6f308740-cdd8-4e00-8285-4974a74bce68/download0a4d114340ad7e38405364286da8b4f8MD52trueAnonymousREADTEXTEloiS_Garcia_etal_IOC_2004.pdf.txtEloiS_Garcia_etal_IOC_2004.pdf.txtExtracted texttext/plain32715https://arca.fiocruz.br/bitstreams/dbb3bb91-c25d-4e95-aec8-e203e06c0c13/download0ccddab09eb51c2a06c69f1b5476c46dMD57falseAnonymousREADTHUMBNAILEloiS_Garcia_etal_IOC_2004.pdf.jpgEloiS_Garcia_etal_IOC_2004.pdf.jpgGenerated Thumbnailimage/jpeg6379https://arca.fiocruz.br/bitstreams/b52a845a-fad3-47a3-8fae-2667c386da7e/downloada9d2972c77b3f6be83c753e3107c30eaMD58falseAnonymousREADicict/445442025-07-31 13:22:46.719open.accessoai:arca.fiocruz.br:icict/44544https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-31T16:22:46Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
title Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
spellingShingle Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
Cunha, J. M. Albuquerque
Microvilosidades
Triatomíneos
Membranas perimicrovilares
Hormônios
Triatomine
Midgut
Perimicrovillar membranes
Hormone
Microvilli
title_short Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
title_full Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
title_fullStr Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
title_full_unstemmed Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
title_sort Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus
author Cunha, J. M. Albuquerque
author_facet Cunha, J. M. Albuquerque
Mello, C. B.
Garcia, Eloi S.
Azambuja, P.
Souza, W. de
Gonzalez, M. S.
Nogueira, N. F. S.
author_role author
author2 Mello, C. B.
Garcia, Eloi S.
Azambuja, P.
Souza, W. de
Gonzalez, M. S.
Nogueira, N. F. S.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, J. M. Albuquerque
Mello, C. B.
Garcia, Eloi S.
Azambuja, P.
Souza, W. de
Gonzalez, M. S.
Nogueira, N. F. S.
dc.subject.other.none.fl_str_mv Microvilosidades
Triatomíneos
Membranas perimicrovilares
Hormônios
topic Microvilosidades
Triatomíneos
Membranas perimicrovilares
Hormônios
Triatomine
Midgut
Perimicrovillar membranes
Hormone
Microvilli
dc.subject.en.none.fl_str_mv Triatomine
Midgut
Perimicrovillar membranes
Hormone
Microvilli
description Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2020-11-19T17:59:22Z
dc.date.available.fl_str_mv 2020-11-19T17:59:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUNHA, J. M. Albuquerque et al. Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 9, p. 815-822, Dec. 2004.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/44544
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762004000800006
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CUNHA, J. M. Albuquerque et al. Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 9, p. 815-822, Dec. 2004.
0074-0276
10.1590/S0074-02762004000800006
1678-8060
url https://arca.fiocruz.br/handle/icict/44544
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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