Apoptosis in a canine model of acute Chagasic myocarditis

Bibliographic Details
Main Author: Zhang, J.
Publication Date: 1999
Other Authors: Andrade, Zilton de Araújo, Yu, Z. X., Andrade, Sonia Gumes, Takeda, K., Sadigursky, Moysés, Ferrans, V. J.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1006/jmcc.1998.0893
Download full: https://arca.fiocruz.br/handle/icict/15311
Summary: National Institutes of Health. Pathology Section. National Heart, Lung & Blood Institute. Bethesda, MD, USA
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spelling Zhang, J.Andrade, Zilton de AraújoYu, Z. X.Andrade, Sonia GumesTakeda, K.Sadigursky, MoysésFerrans, V. J.2016-08-19T19:19:54Z2016-08-19T19:19:54Z1999ZHANG, J. et al. Apoptosis in a canine model of acute Chagasic myocarditis. Journal of Molecular and Cellular Cardiology, v. 31, p. 581–596, 1999.0022-2828https://arca.fiocruz.br/handle/icict/1531110.1006/jmcc.1998.0893engElsevierApoptosis in a canine model of acute Chagasic myocarditisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institutes of Health. Pathology Section. National Heart, Lung & Blood Institute. Bethesda, MD, USAFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFood and Drug Administration. Division of Applied Pharmacology Research. Laurel, MD, USAFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilNational Institutes of Health. Pathology Section. National Heart, Lung & Blood Institute. Bethesda, MD, USAFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFood and Drug Administration. Division of Applied Pharmacology Research. Laurel, MD, USAHistologic, ultrastructural and nick end labeling studies were made to evaluate the occurrence of apoptosis in the hearts of dogs with acute myocarditis due to experimental infection with T. cruzi. The best results for the detection of apoptosis by nick end labeling were obtained by a method combining the use of terminal deoxynucleotidyl transferase, CoCl2 and fluorescein-conjugated deoxyuridine triphosphate, followed by counterstaining of DNA with 4'6-diamidino-2-phenylindole (DAPI) and examination by laser scanning confocal fluorescence microscopy. Apoptosis was found in: (1) cardiac myocytes; (2) endothelial cells of capillaries and venules: (3) immune effector cells, including macrophages, interstitial dendritic cells (antigen-presenting cells) and granular and agranular lymphocytes, and (4) intra- and extracellular forms of T. cruzi. The apoptosis in myocytes and endothelial cells affected cells that were not infected by T. cruzi and was probably caused by the release of toxic mediators of inflammation. The apoptosis of immune effector cells could be related either to the subsidence of inflammation or to modulation (and even failure) of the immune response. The finding of apoptosis in T. cruzi confirms the results of other studies showing that this phenomenon occurs during the differentiation of trypomastigotes in vitro. Thus, apoptosis constitutes an important and multifactorial event in the pathogenesis of acute Chagasic myocarditis.Trypanosoma cruziApoptosisAcute myocarditisDogNick end labelingElectron microscopyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/844fd1af-17ea-49da-b162-aaf0f7960f51/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALZhang Jun Apoptosis in a canine....pdfZhang Jun Apoptosis in a canine....pdfapplication/pdf9995087https://arca.fiocruz.br/bitstreams/80410b5a-81dc-4f25-bfb7-5f5f11ac5194/download597688527ab2449402b5cff7f1ab09f6MD52trueAnonymousREADTEXTZhang Jun Apoptosis in a canine....pdf.txtZhang Jun Apoptosis in a canine....pdf.txtExtracted texttext/plain56528https://arca.fiocruz.br/bitstreams/7c21bd39-95a3-4f55-8093-d081b54e99a0/downloadb55c8a3259eeda4fd50808160708c71aMD55falseAnonymousREADTHUMBNAILZhang Jun Apoptosis in a canine....pdf.jpgZhang Jun Apoptosis in a canine....pdf.jpgGenerated Thumbnailimage/jpeg5268https://arca.fiocruz.br/bitstreams/d7f7e574-ecbb-41d3-ba8b-07fbd82c63ea/download5c4991152686042d75de05e5942a4a87MD56falseAnonymousREADicict/153112025-07-29 21:53:00.956open.accessoai:arca.fiocruz.br:icict/15311https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T00:53Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Apoptosis in a canine model of acute Chagasic myocarditis
title Apoptosis in a canine model of acute Chagasic myocarditis
spellingShingle Apoptosis in a canine model of acute Chagasic myocarditis
Zhang, J.
Trypanosoma cruzi
Apoptosis
Acute myocarditis
Dog
Nick end labeling
Electron microscopy
title_short Apoptosis in a canine model of acute Chagasic myocarditis
title_full Apoptosis in a canine model of acute Chagasic myocarditis
title_fullStr Apoptosis in a canine model of acute Chagasic myocarditis
title_full_unstemmed Apoptosis in a canine model of acute Chagasic myocarditis
title_sort Apoptosis in a canine model of acute Chagasic myocarditis
author Zhang, J.
author_facet Zhang, J.
Andrade, Zilton de Araújo
Yu, Z. X.
Andrade, Sonia Gumes
Takeda, K.
Sadigursky, Moysés
Ferrans, V. J.
author_role author
author2 Andrade, Zilton de Araújo
Yu, Z. X.
Andrade, Sonia Gumes
Takeda, K.
Sadigursky, Moysés
Ferrans, V. J.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zhang, J.
Andrade, Zilton de Araújo
Yu, Z. X.
Andrade, Sonia Gumes
Takeda, K.
Sadigursky, Moysés
Ferrans, V. J.
dc.subject.en.none.fl_str_mv Trypanosoma cruzi
Apoptosis
Acute myocarditis
Dog
Nick end labeling
Electron microscopy
topic Trypanosoma cruzi
Apoptosis
Acute myocarditis
Dog
Nick end labeling
Electron microscopy
description National Institutes of Health. Pathology Section. National Heart, Lung & Blood Institute. Bethesda, MD, USA
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2016-08-19T19:19:54Z
dc.date.available.fl_str_mv 2016-08-19T19:19:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ZHANG, J. et al. Apoptosis in a canine model of acute Chagasic myocarditis. Journal of Molecular and Cellular Cardiology, v. 31, p. 581–596, 1999.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/15311
dc.identifier.issn.none.fl_str_mv 0022-2828
dc.identifier.doi.none.fl_str_mv 10.1006/jmcc.1998.0893
identifier_str_mv ZHANG, J. et al. Apoptosis in a canine model of acute Chagasic myocarditis. Journal of Molecular and Cellular Cardiology, v. 31, p. 581–596, 1999.
0022-2828
10.1006/jmcc.1998.0893
url https://arca.fiocruz.br/handle/icict/15311
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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