Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection

Bibliographic Details
Main Author: Rodolpho, Joice Margareth de Almeida
Publication Date: 2018
Other Authors: Camillo, Luciana, Araújo, Márcio Sobreira Silva, Speziali, Elaine, Reis, Jordana Grazziela Alves Coelho dos, Correia, Ricardo de Oliveira, Neris, Débora Meira, Martins Filho, Olindo Assis, Carvalho, Andréa Teixeira de, Anibal, Fernanda de Freitas
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3389/fimmu.2018.00064
Download full: https://arca.fiocruz.br/handle/icict/28972
Summary: Universidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.
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spelling Rodolpho, Joice Margareth de AlmeidaCamillo, LucianaAraújo, Márcio Sobreira SilvaSpeziali, ElaineReis, Jordana Grazziela Alves Coelho dosCorreia, Ricardo de OliveiraNeris, Débora MeiraMartins Filho, Olindo AssisCarvalho, Andréa Teixeira deAnibal, Fernanda de Freitas2018-09-21T17:54:49Z2018-09-21T17:54:49Z2018RODOLPHO, Joice Margareth de Almeida et al. Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection. Frontiers in Immunology, v. 9, p. 1-14, 2018.1664-3224https://arca.fiocruz.br/handle/icict/2897210.3389/fimmu.2018.00064engFrontiers Research FoundationEosinófilosToxocara canisSLMVAtivaçãoEosinophilToxocara canisSLMVActivationCo-stimulatory moleculesRobust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.Universidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Universidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.Universidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Universidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.Eosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4+ T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of major histocompatibility complex (MHC-II), CD80 and CD86, suggesting they play a role upon Toxocara canis antigen stimulation. In the present study, we evaluated the profile of eosinophils using conventional and image flow cytometry upon experimental T. canis infection. T. canis antigens induced a robust activation on this subset, contributing to the immune responses elicited in the experimental model for T. canis-associated visceral larva migrans syndrome. Data analysis demonstrated that, during murine T. canis infection, eosinophils from peripheral blood, spleen, and bone marrow presented upregulated expression of CD69/MHC-II/CD80/CD86. As opposed to splenic and bone marrow eosinophils, circulating eosinophils had increased expression of activation markers upon T. canis infection. The enhanced connectivity between eosinophils and T-cells in T. canis-infected mice in all three compartments (peripheral blood, spleen, and bone marrow) also supports the hypothesis that eosinophils may adopt a role during T. canis infection. Moreover, in vitro T. canis antigen stimulation resulted in activation and upregulation of co-stimulatory-related molecules by bone marrow-derived eosinophils. Our findings are evidence of activation and upregulation of important activation and co-stimulatory-related molecules in eosinophils and suggest a reshape of activation hierarchy toward eosinophils during experimental T. canis infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://arca.fiocruz.br/bitstreams/921c28af-87c0-465c-9806-089d3a23284a/download9193a7c197bc67acd023525e72a03240MD51falseAnonymousREADORIGINALRobust Phenotypic Activation of Eosinophils.pdfRobust Phenotypic Activation of Eosinophils.pdfapplication/pdf6299777https://arca.fiocruz.br/bitstreams/f6923037-8937-48f7-ad2b-7c213e3619ef/download3c8299cf268ddc88814ed7f91e85c93eMD52trueAnonymousREADTEXTRobust Phenotypic Activation of Eosinophils.pdf.txtRobust Phenotypic Activation of Eosinophils.pdf.txtExtracted texttext/plain243https://arca.fiocruz.br/bitstreams/5408e0e1-2c31-4946-9de4-4067ccd110db/download87daab9c7a23f400a363a415b2fdc367MD55falseAnonymousREADTHUMBNAILRobust Phenotypic Activation of Eosinophils.pdf.jpgRobust Phenotypic Activation of Eosinophils.pdf.jpgGenerated Thumbnailimage/jpeg5074https://arca.fiocruz.br/bitstreams/5cd5df09-30da-4c62-b7ac-2a80e88118fe/downloade3a8b1cc41b256f34f00cd5a0714bcd3MD56falseAnonymousREADicict/289722025-07-29 22:47:28.978open.accessoai:arca.fiocruz.br:icict/28972https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T01:47:28Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
spellingShingle Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
Rodolpho, Joice Margareth de Almeida
Eosinófilos
Toxocara canis
SLMV
Ativação
Eosinophil
Toxocara canis
SLMV
Activation
Co-stimulatory molecules
title_short Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_full Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_fullStr Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_full_unstemmed Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_sort Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
author Rodolpho, Joice Margareth de Almeida
author_facet Rodolpho, Joice Margareth de Almeida
Camillo, Luciana
Araújo, Márcio Sobreira Silva
Speziali, Elaine
Reis, Jordana Grazziela Alves Coelho dos
Correia, Ricardo de Oliveira
Neris, Débora Meira
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
Anibal, Fernanda de Freitas
author_role author
author2 Camillo, Luciana
Araújo, Márcio Sobreira Silva
Speziali, Elaine
Reis, Jordana Grazziela Alves Coelho dos
Correia, Ricardo de Oliveira
Neris, Débora Meira
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
Anibal, Fernanda de Freitas
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodolpho, Joice Margareth de Almeida
Camillo, Luciana
Araújo, Márcio Sobreira Silva
Speziali, Elaine
Reis, Jordana Grazziela Alves Coelho dos
Correia, Ricardo de Oliveira
Neris, Débora Meira
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
Anibal, Fernanda de Freitas
dc.subject.other.none.fl_str_mv Eosinófilos
Toxocara canis
SLMV
Ativação
topic Eosinófilos
Toxocara canis
SLMV
Ativação
Eosinophil
Toxocara canis
SLMV
Activation
Co-stimulatory molecules
dc.subject.en.none.fl_str_mv Eosinophil
Toxocara canis
SLMV
Activation
Co-stimulatory molecules
description Universidade Federal de São Carlos. Departamento de Morfologia e Patologia. Laboratório de Doenças Infecciosas e Inflamatórias. São Carlos, SP, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-21T17:54:49Z
dc.date.available.fl_str_mv 2018-09-21T17:54:49Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODOLPHO, Joice Margareth de Almeida et al. Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection. Frontiers in Immunology, v. 9, p. 1-14, 2018.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/28972
dc.identifier.issn.none.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2018.00064
identifier_str_mv RODOLPHO, Joice Margareth de Almeida et al. Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection. Frontiers in Immunology, v. 9, p. 1-14, 2018.
1664-3224
10.3389/fimmu.2018.00064
url https://arca.fiocruz.br/handle/icict/28972
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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