Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions
| Main Author: | |
|---|---|
| Publication Date: | 2022 |
| Other Authors: | , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3389/fmed.2022.865330 |
| Download full: | https://arca.fiocruz.br/handle/icict/56379 |
Summary: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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Castro, Katherine Kelda Gomes deSilva, Pedro Henrique Lopes daSantos, Luciana Nahar dosLeal, Julia Monteiro PereiraPereira, Mylena Masseno de PinhoAlvim, Iris Maria PeixotoEsquenazi, Danuza2023-01-08T13:01:31Z2023-01-08T13:01:31Z2022CASTRO, Katherine Kelda Gomes de et al. Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions. Frontiers in Medicine, v.9, 865330-p. 1 - 11, July 2022.2298-858Xhttps://arca.fiocruz.br/handle/icict/5637910.3389/fmed.2022.865330engFrontiers MediaHanseníaseReaçõesLinfócitos TTregCitocinasLeprosyReactionsT lymphocytesTregCytokinesDownmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Patologia e Laboratórios. Rio de Janeiro, RJ, Brasil.Leprosy reactions are an acute and systemic manifestation, which occurs suddenly, can be severe and lead leprosy patients to disability. Reactional episodes are observed among half of the multibacillary patients, mainly in borderline lepromatous and lepromatous forms. They may begin at any time during multidrug therapy, and even before the treatment. Physical disabilities, which are the source of extreme suffering and pain for patients, occur in progression of the cellular immune response associated with a reaction and are still poorly understood. Thus, this work aimed to phenotypically and functionally characterize CD4+ and CD8+ Treg cells ex vivo and in response to Mycobacterium leprae (ML). We studied 52 individuals, including 18 newly diagnosed and untreated multibacillary leprosy patients, 19 reactional multibacillary patients (Type I or Type II episodes) and 15 healthy volunteers, included as controls, all residents of the city of Rio de Janeiro. The functional activity and frequencies of these cells were evaluated through multiparametric flow cytometry. In addition, the production of cytokines in supernatant from peripheral blood mononuclear cell cultures was also investigated against ML by enzyme-linked immunosorbent assay. Our results showed a decrease in CD4+TGF-b+ Treg and CD8+ TGF-b+ Treg in leprosy multibacillary patients during both types of reactional episodes. Alterations in the cytokine profile was also observed in Type II reactions, along with upregulation of IL-17 and IL-6 in supernatant. Thus, our study suggests that downregulation of Treg cells is related with both classes of reactional episodes, improving our understanding of immune hyporesponsiveness in multibacillary patients and hyperesponsiveness in both reactions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/63612141-2fc9-42a4-9de9-73de1cea5765/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALKatherineCastro_JuliaMonteiroLeal_etal_IOC_2022.pdfKatherineCastro_JuliaMonteiroLeal_etal_IOC_2022.pdfapplication/pdf1147923https://arca.fiocruz.br/bitstreams/74ab047a-3d0d-43e1-9ed8-eb665eafe1f4/download43a01c0839555e3060adee89c065ea30MD52trueAnonymousREADTEXTKatherineCastro_JuliaMonteiroLeal_etal_IOC_2022.pdf.txtKatherineCastro_JuliaMonteiroLeal_etal_IOC_2022.pdf.txtExtracted texttext/plain59386https://arca.fiocruz.br/bitstreams/5c6993b3-5c48-45ab-8b5a-1139371a998b/download1fc3f654e70bbb7a36fdb56fa86dfebcMD55falseAnonymousREADTHUMBNAILKatherineCastro_JuliaMonteiroLeal_etal_IOC_2022.pdf.jpgKatherineCastro_JuliaMonteiroLeal_etal_IOC_2022.pdf.jpgGenerated Thumbnailimage/jpeg5607https://arca.fiocruz.br/bitstreams/3d14e490-16ce-4b4f-a382-502c7d5bfa46/download175a48bcf4837fac78cb491a508e2887MD56falseAnonymousREADicict/563792025-07-30 01:21:57.552open.accessoai:arca.fiocruz.br:icict/56379https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T04:21:57Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| title |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| spellingShingle |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions Castro, Katherine Kelda Gomes de Hanseníase Reações Linfócitos T Treg Citocinas Leprosy Reactions T lymphocytes Treg Cytokines |
| title_short |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| title_full |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| title_fullStr |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| title_full_unstemmed |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| title_sort |
Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions |
| author |
Castro, Katherine Kelda Gomes de |
| author_facet |
Castro, Katherine Kelda Gomes de Silva, Pedro Henrique Lopes da Santos, Luciana Nahar dos Leal, Julia Monteiro Pereira Pereira, Mylena Masseno de Pinho Alvim, Iris Maria Peixoto Esquenazi, Danuza |
| author_role |
author |
| author2 |
Silva, Pedro Henrique Lopes da Santos, Luciana Nahar dos Leal, Julia Monteiro Pereira Pereira, Mylena Masseno de Pinho Alvim, Iris Maria Peixoto Esquenazi, Danuza |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Castro, Katherine Kelda Gomes de Silva, Pedro Henrique Lopes da Santos, Luciana Nahar dos Leal, Julia Monteiro Pereira Pereira, Mylena Masseno de Pinho Alvim, Iris Maria Peixoto Esquenazi, Danuza |
| dc.subject.other.none.fl_str_mv |
Hanseníase Reações Linfócitos T Treg Citocinas |
| topic |
Hanseníase Reações Linfócitos T Treg Citocinas Leprosy Reactions T lymphocytes Treg Cytokines |
| dc.subject.en.none.fl_str_mv |
Leprosy Reactions T lymphocytes Treg Cytokines |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2022 |
| dc.date.issued.fl_str_mv |
2022 |
| dc.date.accessioned.fl_str_mv |
2023-01-08T13:01:31Z |
| dc.date.available.fl_str_mv |
2023-01-08T13:01:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
CASTRO, Katherine Kelda Gomes de et al. Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions. Frontiers in Medicine, v.9, 865330-p. 1 - 11, July 2022. |
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https://arca.fiocruz.br/handle/icict/56379 |
| dc.identifier.issn.none.fl_str_mv |
2298-858X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmed.2022.865330 |
| identifier_str_mv |
CASTRO, Katherine Kelda Gomes de et al. Downmodulation of Regulatory T Cells Producing TGF-b Participates in Pathogenesis of Leprosy Reactions. Frontiers in Medicine, v.9, 865330-p. 1 - 11, July 2022. 2298-858X 10.3389/fmed.2022.865330 |
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Frontiers Media |
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Frontiers Media |
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