SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia
| Main Author: | |
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| Publication Date: | 2023 |
| Other Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1177/11779322231186477 |
| Download full: | https://arca.fiocruz.br/handle/icict/68214 |
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Sgorlon, GabriellaRoca, Tárcio PeixotoPassos-Silva, Ana MaisaCustódio, Márlon Grégori FloresQueiroz, Jackson Alves da SilvaSilva, André Luiz Ferreira daTeixeira, Karolaine SantosBatista, Flávia SerranoSalcedo, Juan Miguel VillalobosRampazzo, Rita de Cassia P.Naveca, Felipe GomesVieira, Deusilene2025-01-23T14:42:20Z2025-01-23T14:42:20Z2023SGORLON, Gabriella et al. SARS-CoV-2 Spike Protein Mutations in Different Variants: a comparison between vaccinated and unvaccinated population in western Amazonia. Bioinformatics and Biology Insights, v. 17, p. 1-9, 14 Jul. 2023.1177-9322https://arca.fiocruz.br/handle/icict/6821410.1177/11779322231186477FIOCRUZ/RO, DECIT, FAPERO,Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Governo de Rondônia. Agência Estadual de Vigilância em Saúde de Rondônia. Coordenação Estadual do COVID-19. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Paraná. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Fiocruz Amazônia. Instituto Leônidas e Maria Deane. Laboratório de Virologia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.The increased transmissibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has generated variants of concern (VOCs) throughout the pandemic, responsible for waves of cases worldwide. To monitor mutations in the S gene of SARS-CoV-2 in different variants, we evaluated 1497 individuals with COVID-19 in western Amazonia in the period April 2021 to July 2022. The epidemiological and clinical data of the individuals were collected; subsequently, the samples were extracted using a commercial kit, the viral load was assessed, and viral genomes were sequenced. We analyzed the quality and mutations of the genomes and maximum likelihood phylogenetic inference. However, 3 main clusters were observed, referring to Gamma (52.91%), Delta (24.38%), and Omicron (20.38%) VOCs with wide distribution in all health regions of the Rondônia state. Regarding the vaccination profile, there was a higher percentage of unvaccinated and partially vaccinated individuals, with more representatives by the Gamma variant. A total of 1412 sequences were suitable for mutation analysis in the S gene region. The Omicron VOC showed 38 mutations, with the Delta and Gamma variants with 16 and 17, respectively. The VOC Omicron and Gamma shared 4 mutations E484K, H655Y, N501Y, and N679K with high frequency, and Delta and Omicron 2 mutations (T478K and T95I). Regarding the comparison between the frequency of mutations for each variant concerning the vaccination groups, there were no changes in mutations for each group. In conclusion, the study showed a temporal increase in mutations and subvariants for characterized strains. 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| dc.title.none.fl_str_mv |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| title |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| spellingShingle |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia Sgorlon, Gabriella SARS-CoV-2 Variants Sequences Mutations Vaccination SARS-CoV-2 Mutation Vaccination |
| title_short |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| title_full |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| title_fullStr |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| title_full_unstemmed |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| title_sort |
SARS-CoV-2 Spike Protein Mutations in Different Variants: A Comparison Between Vaccinated and Unvaccinated Population in Western Amazonia |
| author |
Sgorlon, Gabriella |
| author_facet |
Sgorlon, Gabriella Roca, Tárcio Peixoto Passos-Silva, Ana Maisa Custódio, Márlon Grégori Flores Queiroz, Jackson Alves da Silva Silva, André Luiz Ferreira da Teixeira, Karolaine Santos Batista, Flávia Serrano Salcedo, Juan Miguel Villalobos Rampazzo, Rita de Cassia P. Naveca, Felipe Gomes Vieira, Deusilene |
| author_role |
author |
| author2 |
Roca, Tárcio Peixoto Passos-Silva, Ana Maisa Custódio, Márlon Grégori Flores Queiroz, Jackson Alves da Silva Silva, André Luiz Ferreira da Teixeira, Karolaine Santos Batista, Flávia Serrano Salcedo, Juan Miguel Villalobos Rampazzo, Rita de Cassia P. Naveca, Felipe Gomes Vieira, Deusilene |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sgorlon, Gabriella Roca, Tárcio Peixoto Passos-Silva, Ana Maisa Custódio, Márlon Grégori Flores Queiroz, Jackson Alves da Silva Silva, André Luiz Ferreira da Teixeira, Karolaine Santos Batista, Flávia Serrano Salcedo, Juan Miguel Villalobos Rampazzo, Rita de Cassia P. Naveca, Felipe Gomes Vieira, Deusilene |
| dc.subject.en.none.fl_str_mv |
SARS-CoV-2 Variants Sequences Mutations Vaccination |
| topic |
SARS-CoV-2 Variants Sequences Mutations Vaccination SARS-CoV-2 Mutation Vaccination |
| dc.subject.decs.none.fl_str_mv |
SARS-CoV-2 Mutation Vaccination |
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FIOCRUZ/RO, DECIT, FAPERO, |
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2023 |
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2023 |
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2025-01-23T14:42:20Z |
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2025-01-23T14:42:20Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
SGORLON, Gabriella et al. SARS-CoV-2 Spike Protein Mutations in Different Variants: a comparison between vaccinated and unvaccinated population in western Amazonia. Bioinformatics and Biology Insights, v. 17, p. 1-9, 14 Jul. 2023. |
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https://arca.fiocruz.br/handle/icict/68214 |
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1177-9322 |
| dc.identifier.doi.none.fl_str_mv |
10.1177/11779322231186477 |
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SGORLON, Gabriella et al. SARS-CoV-2 Spike Protein Mutations in Different Variants: a comparison between vaccinated and unvaccinated population in western Amazonia. Bioinformatics and Biology Insights, v. 17, p. 1-9, 14 Jul. 2023. 1177-9322 10.1177/11779322231186477 |
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SAGE Publications |
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SAGE Publications |
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