A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods

Bibliographic Details
Main Author: Viana, Débora Leal
Publication Date: 2022
Other Authors: Alladagbin, Dona Jeanne, Santos, Washington L. C. dos, Figueira, Cláudio Pereira
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: doi.org/10.1186/s12882-021-02634-1
Download full: https://arca.fiocruz.br/handle/icict/51677
Summary: Referral Liver & Kidney Pathology Diagnosis, Instituto Gonçalo Moniz - FIOCRUZ.
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spelling Viana, Débora LealAlladagbin, Dona JeanneSantos, Washington L. C. dosFigueira, Cláudio Pereira2022-03-10T17:13:19Z2022-03-10T17:13:19Z2022Viana, Débora Leal. A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods. BMC Nephrology, v. 23, n. 23, p. 1-7, 21471-2369https://arca.fiocruz.br/handle/icict/51677doi.org/10.1186/s12882-021-02634-1Referral Liver & Kidney Pathology Diagnosis, Instituto Gonçalo Moniz - FIOCRUZ.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Introduction: Here we report estimates of glomerular basement membrane (GBM) thickness in the Brazilian population performed using direct (DM) and orthogonal interception methods (OIM), and comment on potential sources of variation among estimates made by different laboratories. Methodology: A total of 38 patients, ranging from 3 to 78 years of age, 26 (68%) males and 12 (32%) females, were submitted to kidney biopsy procedures for renal disease diagnosis. Glomeruli were diagnosed with minor histological changes by conventional, immunofluorescence and electron microscopy. GBM thickness was estimated using both DM and OIM methods. Results: Estimates of GBM thickness obtained using DM were higher than those obtained by OIM. However, the application of a correction for non-perpendicular membrane sectioning to DM estimates yielded similar results to those obtained under OIM. The estimated GMB thickness using DM after correction was 289 + 44 nm, versus 287 + 48 nm by OIM. No statistically significant differences were detected in GMB thickness, nor with respect to patient age or sex. Conclusions: GBM thickness in the studied Brazilian population measured approximately 290 nm. The application of criteria for estimating the shortest distance between the endothelial and podocyte cell membranes with correction for non-perpendicular membrane sectioning can increase the accuracy of GBM thickness estimates using DM and OIM.engBMCMembrana Basal GlomerularEstudo ComparativoGlomerular Basement MembraneComparative StudyMembrana Basal GlomerularEstudio ComparativoMembrane basale glomérulaireÉtude comparativeA comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/06da3d3a-3824-4f15-b52c-684cc6d38255/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALViana, D. L. 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dc.title.none.fl_str_mv A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
title A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
spellingShingle A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
Viana, Débora Leal
Membrana Basal Glomerular
Estudo Comparativo
Glomerular Basement Membrane
Comparative Study
Membrana Basal Glomerular
Estudio Comparativo
Membrane basale glomérulaire
Étude comparative
title_short A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
title_full A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
title_fullStr A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
title_full_unstemmed A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
title_sort A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods
author Viana, Débora Leal
author_facet Viana, Débora Leal
Alladagbin, Dona Jeanne
Santos, Washington L. C. dos
Figueira, Cláudio Pereira
author_role author
author2 Alladagbin, Dona Jeanne
Santos, Washington L. C. dos
Figueira, Cláudio Pereira
author2_role author
author
author
dc.contributor.author.fl_str_mv Viana, Débora Leal
Alladagbin, Dona Jeanne
Santos, Washington L. C. dos
Figueira, Cláudio Pereira
dc.subject.other.none.fl_str_mv Membrana Basal Glomerular
Estudo Comparativo
topic Membrana Basal Glomerular
Estudo Comparativo
Glomerular Basement Membrane
Comparative Study
Membrana Basal Glomerular
Estudio Comparativo
Membrane basale glomérulaire
Étude comparative
dc.subject.en.none.fl_str_mv Glomerular Basement Membrane
Comparative Study
dc.subject.es.none.fl_str_mv Membrana Basal Glomerular
Estudio Comparativo
dc.subject.fr.none.fl_str_mv Membrane basale glomérulaire
Étude comparative
description Referral Liver & Kidney Pathology Diagnosis, Instituto Gonçalo Moniz - FIOCRUZ.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-10T17:13:19Z
dc.date.available.fl_str_mv 2022-03-10T17:13:19Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Viana, Débora Leal. A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods. BMC Nephrology, v. 23, n. 23, p. 1-7, 2
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/51677
dc.identifier.issn.none.fl_str_mv 1471-2369
dc.identifier.doi.none.fl_str_mv doi.org/10.1186/s12882-021-02634-1
identifier_str_mv Viana, Débora Leal. A comparative study of human glomerular basement membrane thickness using direct measurement and orthogonal intercept methods. BMC Nephrology, v. 23, n. 23, p. 1-7, 2
1471-2369
doi.org/10.1186/s12882-021-02634-1
url https://arca.fiocruz.br/handle/icict/51677
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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