Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
| Main Author: | |
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| Publication Date: | 2021 |
| Other Authors: | , , , , , , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.toxlet.2020.11.019 |
| Download full: | https://arca.fiocruz.br/handle/icict/45498 |
Summary: | Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil. |
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Simões, Letícia S.Alves, Quiara L.Camargo, Samuel B.Araújo, Fênix A.Hora, Viviane R. S.Jesus, Rafael L. C.Barreto, Breno C.Macambira, Simone G.Soares, Milena B. P.Meira, Cássio S.Aguiar, Márcio C.Couto, Ricardo D.Lomonte, BrunoMenezes Filho, José EvaldoCruz, Jader S.Santos, Marcos A. VannierSilva, Luciana S. Casais eSilva, Darizy F.2021-01-04T17:02:40Z2021-01-04T17:02:40Z2021SIMÕES, Letícia O. et al. Cardiac effect induced by Crotalus durissus venom: Morphofunctional evidence and mechanism of action. Toxicology Letters, v. 337, p. 121-133, 2021.0378-4274https://arca.fiocruz.br/handle/icict/4549810.1016/j.toxlet.2020.11.019engElsevierCrotalus durissus cascavellaCardiotoxicidadeFibrilaçãoCrotalus durissus cascavellaCardiotoxicityAtrialApêndice AtrialCardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of actioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Departamento de Bioquímica e Biofísica. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biomorfologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Análise Clínica e Toxicológica. Salvador, BA, Brasil.Universidade da Costa Rica. Faculdade de Microbiologia. Instituto Clodomiro Picado. San José, Costa Rica.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Envenoming, resulting from snake bites, is a global public health problem. The present study was undertaken to investigate the influence of Crotalus durissus cascavella (Cdcas) venom on cardiac activity and the mechanisms of action underlying its effect. To investigate the inotropic and chronotropic effects induced by Cdcas, studies were performed on the left and right atria. A series of tests were conducted to investigate whether the negative inotropic effect, induced by Cdcas, was related to cardiac damage. Cdcas venom (0.1-30 μg/mL) elicited a significant negative inotropic effect. The addition of Cdcas crude venom (7.5, 15 and 30 μg/mL) did not induce significant alterations in cell proliferation, nor in the enzymatic activity of total-CK and CKMB. Ultrastructural evaluation demonstrated that cardiac cells from isoproterenol and Cdcas groups revealed discreet swelling and displaced intermyofibrillar mitochondria with disorganization of the cristae. No change was observed in cardiac electrical activity in perfused isolated rat hearts with Cdcas. In addition, Cdcas reduced contractility in isolated cardiomyocytes from the rat left ventricle. The negative inotropic effect of Cdcas was reduced by l-NAME (100 μM), PTIO (100 μM), ODQ (10 μM) and KT5823 (1 μM), suggesting the participation of NO/cGMP/PKG pathway due to Cdcas. In non-anesthetized rats, Cdcas induced hypotension followed by bradycardia, the latter was also observed by ECG (anesthetized animals). Our results suggest that the negative inotropic effect induced by Cdcas venom is unrelated to cardiac toxicity, at least, at the concentrations tested; and occurs through of NO/cGMP/PKG pathway, likely leading to hypotension and bradycardia when administered in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/80efdea5-6129-48f3-93b4-a5c14fce4c7d/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALMarcosVannier_santos_etal_IOC_2020.pdfMarcosVannier_santos_etal_IOC_2020.pdfapplication/pdf5333824https://arca.fiocruz.br/bitstreams/8efa9a36-f89d-4da4-9f68-7beb6bae2500/download5206cea0145df8695ab7601a3a488dceMD52trueAnonymousREAD2025-01-01TEXTMarcosVannier_santos_etal_IOC_2020.pdf.txtMarcosVannier_santos_etal_IOC_2020.pdf.txtExtracted texttext/plain77085https://arca.fiocruz.br/bitstreams/497724c6-32ef-4b1a-a70e-f3d8f6bc9bc1/download447dd9d6da204433809587f02ecf73f5MD57falseAnonymousREAD2025-01-01THUMBNAILMarcosVannier_santos_etal_IOC_2020.pdf.jpgMarcosVannier_santos_etal_IOC_2020.pdf.jpgGenerated Thumbnailimage/jpeg5546https://arca.fiocruz.br/bitstreams/7ae9e60e-b9d2-43e4-a076-9e09e548c171/download03b1fa46a0769998beb9e382ae323b6dMD58falseAnonymousREAD2025-01-01icict/454982025-07-29 18:41:49.882open.accessoai:arca.fiocruz.br:icict/45498https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T21:41:49Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| title |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| spellingShingle |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action Simões, Letícia S. Crotalus durissus cascavella Cardiotoxicidade Fibrilação Crotalus durissus cascavella Cardiotoxicity Atrial Apêndice Atrial |
| title_short |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| title_full |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| title_fullStr |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| title_full_unstemmed |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| title_sort |
Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action |
| author |
Simões, Letícia S. |
| author_facet |
Simões, Letícia S. Alves, Quiara L. Camargo, Samuel B. Araújo, Fênix A. Hora, Viviane R. S. Jesus, Rafael L. C. Barreto, Breno C. Macambira, Simone G. Soares, Milena B. P. Meira, Cássio S. Aguiar, Márcio C. Couto, Ricardo D. Lomonte, Bruno Menezes Filho, José Evaldo Cruz, Jader S. Santos, Marcos A. Vannier Silva, Luciana S. Casais e Silva, Darizy F. |
| author_role |
author |
| author2 |
Alves, Quiara L. Camargo, Samuel B. Araújo, Fênix A. Hora, Viviane R. S. Jesus, Rafael L. C. Barreto, Breno C. Macambira, Simone G. Soares, Milena B. P. Meira, Cássio S. Aguiar, Márcio C. Couto, Ricardo D. Lomonte, Bruno Menezes Filho, José Evaldo Cruz, Jader S. Santos, Marcos A. Vannier Silva, Luciana S. Casais e Silva, Darizy F. |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Simões, Letícia S. Alves, Quiara L. Camargo, Samuel B. Araújo, Fênix A. Hora, Viviane R. S. Jesus, Rafael L. C. Barreto, Breno C. Macambira, Simone G. Soares, Milena B. P. Meira, Cássio S. Aguiar, Márcio C. Couto, Ricardo D. Lomonte, Bruno Menezes Filho, José Evaldo Cruz, Jader S. Santos, Marcos A. Vannier Silva, Luciana S. Casais e Silva, Darizy F. |
| dc.subject.other.none.fl_str_mv |
Crotalus durissus cascavella Cardiotoxicidade Fibrilação |
| topic |
Crotalus durissus cascavella Cardiotoxicidade Fibrilação Crotalus durissus cascavella Cardiotoxicity Atrial Apêndice Atrial |
| dc.subject.en.none.fl_str_mv |
Crotalus durissus cascavella Cardiotoxicity Atrial |
| dc.subject.decs.none.fl_str_mv |
Apêndice Atrial |
| description |
Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil. |
| publishDate |
2021 |
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2021-01-04T17:02:40Z |
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2021-01-04T17:02:40Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SIMÕES, Letícia O. et al. Cardiac effect induced by Crotalus durissus venom: Morphofunctional evidence and mechanism of action. Toxicology Letters, v. 337, p. 121-133, 2021. |
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https://arca.fiocruz.br/handle/icict/45498 |
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0378-4274 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.toxlet.2020.11.019 |
| identifier_str_mv |
SIMÕES, Letícia O. et al. Cardiac effect induced by Crotalus durissus venom: Morphofunctional evidence and mechanism of action. Toxicology Letters, v. 337, p. 121-133, 2021. 0378-4274 10.1016/j.toxlet.2020.11.019 |
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openAccess |
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Elsevier |
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Elsevier |
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