Viability study of a multiplex diagnostic platform for Chagas disease
| Main Author: | |
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| Publication Date: | 2009 |
| Other Authors: | , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1590/S0074-02762009000900019 |
| Download full: | https://arca.fiocruz.br/handle/icict/64023 |
Summary: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Foti, LeonardoFonseca, Bruna de Paula Fonseca eNascimento, Lilian DiasMarques, Christiane de Fatima SilvaSilva, Edmilson Domingos daDuarte, Cesar Augusto BarrosProbst, Christian M.Goldenberg, SamuelPinto, Antônio GomesKrieger, Marco Aurélio2024-05-13T15:44:25Z2024-05-13T15:44:25Z2009FOTI, Leonardo et al. Viability study of a multiplex diagnostic platform for Chagas disease. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, suppl. 1, p. 136-141, Jul. 2009.0074-0276https://arca.fiocruz.br/handle/icict/6402310.1590/S0074-02762009000900019engFiocruz/IOCViability study of a multiplex diagnostic platform for Chagas diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.A new multiplex assay platform was evaluated to detect Trypanosoma cruzi infection using the recombinant antigens CRA, FRA, CRAFRA fusion and parasite lysate. The antigens presented different sensitivity and specificity in a singleplex test when compared to a serial dilution of two pools comprising 10 positive serum samples and one pool of 10 negative samples. The recombinant protein CRA presented lower sensitivity (55%) in contrast to the 100% specificity and sensitivity of FRA, CRAFRA and T. cruzi lysate. These antigens also showed good results in a duplex test and the duplex test with CRAFRA/T. cruzi lysate showed better performance with 100% specificity and sensitivity, as well as a lower cut-off value in comparison to the other duplex test, FRA/T. cruzi lysate. Hence, when the antigens were used in duplex format, both tests showed decreased cut-off values and no interference between different bead sets, resulting in increasing sensitivity and specificity. The results of these multiplex tests show that they could be an alternative to singleplex detection for Chagas disease, and also indicate the necessity of using multiplex diagnostic tools to increase the sensitivity and specificity for diagnostic tests. Emerging data from the T. cruzi genome and from its ORFeome project will also allow the identification of new antigens for this disease detection application.Chagas diseaseDiagnosticMultiplexBead-based assayTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://arca.fiocruz.br/bitstreams/5bb5f162-2cd1-44fa-92af-72148e5070c2/download8a4605be74aa9ea9d79846c1fba20a33MD51falseAnonymousREADORIGINALva_Foti_Leonardo_etal_biomanguinhos_2009.pdfva_Foti_Leonardo_etal_biomanguinhos_2009.pdfapplication/pdf802035https://arca.fiocruz.br/bitstreams/fdf6d9f8-77f6-4d70-b38b-f314ad768525/download643b5e0db66c7247fa30b0b2f3dcf14eMD53trueAnonymousREADTEXTva_Foti_Leonardo_etal_biomanguinhos_2009.pdf.txtva_Foti_Leonardo_etal_biomanguinhos_2009.pdf.txtExtracted texttext/plain30355https://arca.fiocruz.br/bitstreams/6944c1ee-f6b2-4c7c-af8f-3a24015eafed/download10673f31b98432b8ad3e22d91c69f687MD58falseAnonymousREADTHUMBNAILva_Foti_Leonardo_etal_biomanguinhos_2009.pdf.jpgva_Foti_Leonardo_etal_biomanguinhos_2009.pdf.jpgGenerated Thumbnailimage/jpeg5953https://arca.fiocruz.br/bitstreams/a484952a-f7c7-4530-8729-b99f77b4ace1/download6957fee27cd7ca2413de5b5194a915e2MD59falseAnonymousREADicict/640232025-07-29 19:08:10.544open.accessoai:arca.fiocruz.br:icict/64023https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T22:08:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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 |
| dc.title.none.fl_str_mv |
Viability study of a multiplex diagnostic platform for Chagas disease |
| title |
Viability study of a multiplex diagnostic platform for Chagas disease |
| spellingShingle |
Viability study of a multiplex diagnostic platform for Chagas disease Foti, Leonardo Chagas disease Diagnostic Multiplex Bead-based assay Trypanosoma cruzi |
| title_short |
Viability study of a multiplex diagnostic platform for Chagas disease |
| title_full |
Viability study of a multiplex diagnostic platform for Chagas disease |
| title_fullStr |
Viability study of a multiplex diagnostic platform for Chagas disease |
| title_full_unstemmed |
Viability study of a multiplex diagnostic platform for Chagas disease |
| title_sort |
Viability study of a multiplex diagnostic platform for Chagas disease |
| author |
Foti, Leonardo |
| author_facet |
Foti, Leonardo Fonseca, Bruna de Paula Fonseca e Nascimento, Lilian Dias Marques, Christiane de Fatima Silva Silva, Edmilson Domingos da Duarte, Cesar Augusto Barros Probst, Christian M. Goldenberg, Samuel Pinto, Antônio Gomes Krieger, Marco Aurélio |
| author_role |
author |
| author2 |
Fonseca, Bruna de Paula Fonseca e Nascimento, Lilian Dias Marques, Christiane de Fatima Silva Silva, Edmilson Domingos da Duarte, Cesar Augusto Barros Probst, Christian M. Goldenberg, Samuel Pinto, Antônio Gomes Krieger, Marco Aurélio |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Foti, Leonardo Fonseca, Bruna de Paula Fonseca e Nascimento, Lilian Dias Marques, Christiane de Fatima Silva Silva, Edmilson Domingos da Duarte, Cesar Augusto Barros Probst, Christian M. Goldenberg, Samuel Pinto, Antônio Gomes Krieger, Marco Aurélio |
| dc.subject.en.none.fl_str_mv |
Chagas disease Diagnostic Multiplex Bead-based assay Trypanosoma cruzi |
| topic |
Chagas disease Diagnostic Multiplex Bead-based assay Trypanosoma cruzi |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
| publishDate |
2009 |
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2009 |
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2024-05-13T15:44:25Z |
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2024-05-13T15:44:25Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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FOTI, Leonardo et al. Viability study of a multiplex diagnostic platform for Chagas disease. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, suppl. 1, p. 136-141, Jul. 2009. |
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https://arca.fiocruz.br/handle/icict/64023 |
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0074-0276 |
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10.1590/S0074-02762009000900019 |
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FOTI, Leonardo et al. Viability study of a multiplex diagnostic platform for Chagas disease. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, suppl. 1, p. 136-141, Jul. 2009. 0074-0276 10.1590/S0074-02762009000900019 |
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Fiocruz/IOC |
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