Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil

Bibliographic Details
Main Author: Gallimore, C. I.
Publication Date: 2004
Other Authors: Barreiros, M. A. B., Brown, D. W. G., Nascimento, J. P., Leite, J. P. G.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0100-879X2004000300005
Download full: https://arca.fiocruz.br/handle/icict/63999
Summary: Enteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory. Specialist and Reference Microbiology Division. Health Protection Agency. Colindale, London, UK.
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spelling Gallimore, C. I.Barreiros, M. A. B.Brown, D. W. G.Nascimento, J. P.Leite, J. P. G.2024-05-09T18:03:28Z2024-05-09T18:03:28Z2004GALLIMORE, C. I. et al. Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil. Brazilian Journal of Medical and Biological Research, v. 37, n. 3, p. 321-326, Mar. 2004.0100-879Xhttps://arca.fiocruz.br/handle/icict/6399910.1590/S0100-879X2004000300005engAssociação Brasileira de Divulgação CientíficaNoroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEnteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory. Specialist and Reference Microbiology Division. Health Protection Agency. Colindale, London, UK.Universidade do Vale do Itajaí. Itajaí, SC, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Enteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory. Specialist and Reference Microbiology Division. Health Protection Agency. Colindale, London, UK.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Noroviruses (Norwalk-like viruses) are an important cause of gastroenteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical wards, and in hotel and restaurant settings. Food-borne outbreaks have also occurred following consumption of contaminated oysters. This study describes the application of a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA polymerasecoding region of the norovirus genome, and DNA sequencing for the detection and preliminary characterisation of noroviruses in outbreaks of gastroenteritis in children in Brazil. The outbreak samples were collected from children <5 years of age at the Bertha Lutz children's day care facility at Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, that occurred between 1996 and 1998, where no pathogen had been identified. At the Bertha Lutz day care center facility, only Fiocruz's employee children are provided for, and they come from different social, economic and cultural backgrounds. Three distinct genogroup II strains were detected in three outbreaks in 1997/98 and were most closely related to genotypes GII-3 (Mexico virus) and GII4 (Grimsby virus), both of which have been detected in paediatric and adult outbreaks of gastroenteritis worldwide.NorovirusesDay Care CenterGastroenteritisGenotypesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALva_Gallimore_C. I._etal_biomanguinhos_2004.pdfva_Gallimore_C. I._etal_biomanguinhos_2004.pdfapplication/pdf512601https://arca.fiocruz.br/bitstreams/8a9c5a06-b964-421f-9a9f-d4d88458d3b4/downloadc08f2bb5cfe016baf38a33c34b1905f9MD52trueAnonymousREADLICENSElicense.txttext/plain1748https://arca.fiocruz.br/bitstreams/ef41f847-d09d-4caa-a28b-a2f9c379c9ff/download8a4605be74aa9ea9d79846c1fba20a33MD51falseAnonymousREADTEXTva_Gallimore_C. I._etal_biomanguinhos_2004.pdf.txtva_Gallimore_C. 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dc.title.none.fl_str_mv Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
title Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
spellingShingle Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
Gallimore, C. I.
Noroviruses
Day Care Center
Gastroenteritis
Genotypes
title_short Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
title_full Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
title_fullStr Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
title_full_unstemmed Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
title_sort Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
author Gallimore, C. I.
author_facet Gallimore, C. I.
Barreiros, M. A. B.
Brown, D. W. G.
Nascimento, J. P.
Leite, J. P. G.
author_role author
author2 Barreiros, M. A. B.
Brown, D. W. G.
Nascimento, J. P.
Leite, J. P. G.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gallimore, C. I.
Barreiros, M. A. B.
Brown, D. W. G.
Nascimento, J. P.
Leite, J. P. G.
dc.subject.en.none.fl_str_mv Noroviruses
Day Care Center
Gastroenteritis
Genotypes
topic Noroviruses
Day Care Center
Gastroenteritis
Genotypes
description Enteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory. Specialist and Reference Microbiology Division. Health Protection Agency. Colindale, London, UK.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2024-05-09T18:03:28Z
dc.date.available.fl_str_mv 2024-05-09T18:03:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GALLIMORE, C. I. et al. Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil. Brazilian Journal of Medical and Biological Research, v. 37, n. 3, p. 321-326, Mar. 2004.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/63999
dc.identifier.issn.none.fl_str_mv 0100-879X
dc.identifier.doi.none.fl_str_mv 10.1590/S0100-879X2004000300005
identifier_str_mv GALLIMORE, C. I. et al. Noroviruses associated with acute gastroenteritis in a children’s day care facility in Rio de Janeiro, Brazil. Brazilian Journal of Medical and Biological Research, v. 37, n. 3, p. 321-326, Mar. 2004.
0100-879X
10.1590/S0100-879X2004000300005
url https://arca.fiocruz.br/handle/icict/63999
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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