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The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors

Bibliographic Details
Main Author: Silveira, Amanda Cardoso de Oliveira
Publication Date: 2015
Other Authors: Santana, Márcio Antônio Portugal, Ribeiro, Isabela Gomes, Chaves, Daniel Gonçalves, Martins Filho, Olindo Assis
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/j.molbiopara.2015.02.001
Download full: https://arca.fiocruz.br/handle/icict/12645
Summary: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.
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spelling Silveira, Amanda Cardoso de OliveiraSantana, Márcio Antônio PortugalRibeiro, Isabela GomesChaves, Daniel GonçalvesMartins Filho, Olindo Assis2016-01-28T13:16:38Z2016-01-28T13:16:38Z2015SILVEIRA, Amanda Cardoso de Oliveira et al. The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors. BMC Hematology, vol. 15, n. 1, p. 1, 20152052-1839https://arca.fiocruz.br/handle/icict/1264510.1016/j.molbiopara.2015.02.001engBioMed CentralThe IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Hemominas. Serviço de Pesquisa. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil/ Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Hemominas. Serviço de Pesquisa. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Background: Hemophilia A (HA) is an X-linked inherited bleeding disorder, resulting from a qualitative or quantitative deficiency of clotting factor VIII (FVIII). Antibodies against FVIII, also called inhibitors, block the procoagulant activity of FVIII; thus, impairing hemostatic activity in patients with HA. The exact mechanism underlying the immunological events behind the development of inhibitors remains unknown. This study aimed to understand immune response to FVIII in patients with HA who were either positive [HAα-FVIII(+)] or negative [HAα-FVIII(−)] for inhibitors. Methods: Cytokine profiles [interferon-γ (IFN − γ), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), IL-5, and IL-10] of innate and adaptive immune cells present in the peripheral blood of participants were characterized. Results: Presence of inhibitors was significantly associated with decreased frequencies of TNF-α-positive monocytes and neutrophils, IL-5-positive monocytes, IL-4-positive neutrophils, and increased frequencies of IL-10-positive neutrophils and T cells. T cells from HAα-FVIII(−) patients expressed increased levels of almost all cytokines. In contrast, HAα-FVIII(+) patients showed lower levels of all cytokines in CD4+ and CD8+ T cells, except IL-10. B cells from HAα-FVIII(−) patients expressed increased levels of IL-4 while those from HAα-FVIII(+) patients expressed increased levels of IL-10. Conclusions: The global cytokine profiles of innate and adaptive immune cells showed an anti-inflammatory/regulatory pattern in HAα-FVIII(+) patients and a mixed pattern, with a bias toward inflammatory cytokine profile, in HAα-FVIII(−) patients. The occurrence of these profiles seems to be associated with presence FVIII inhibitors.Immune regulationIntracellular cytokinestaining Cytokineprofile FVIIIinhibitors Hemophilia Ainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/40290c3d-a1b3-4101-9987-7a658313502e/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALThe IL-10 polarized cytokine pattern in innate.pdfThe IL-10 polarized cytokine pattern in innate.pdfapplication/pdf5676092https://arca.fiocruz.br/bitstreams/7d599c1e-b0ac-49c6-94a5-1e5ad2b4aa0d/downloadd2026ecc1b245ff944ff2fbe153256a6MD52trueAnonymousREADTEXTThe IL-10 polarized cytokine pattern in innate.pdf.txtThe IL-10 polarized cytokine pattern in innate.pdf.txtExtracted texttext/plain508https://arca.fiocruz.br/bitstreams/8415fe8f-b320-4c97-b49e-5ddadf35695c/downloadd0135ae5efae4596fdbee92c5e7a707bMD55falseAnonymousREADTHUMBNAILThe IL-10 polarized cytokine pattern in innate.pdf.jpgThe IL-10 polarized cytokine pattern in innate.pdf.jpgGenerated Thumbnailimage/jpeg5407https://arca.fiocruz.br/bitstreams/acdabf17-69bf-4d1a-9e80-4639d0e8496a/downloada2a971b62163c84dad363a6faaff6d2bMD56falseAnonymousREADicict/126452025-07-29 23:19:21.258open.accessoai:arca.fiocruz.br:icict/12645https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T02:19:21Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.none.fl_str_mv The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
title The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
spellingShingle The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
Silveira, Amanda Cardoso de Oliveira
Immune regulation
Intracellular cytokine
staining Cytokine
profile FVIII
inhibitors Hemophilia A
title_short The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
title_full The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
title_fullStr The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
title_full_unstemmed The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
title_sort The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors
author Silveira, Amanda Cardoso de Oliveira
author_facet Silveira, Amanda Cardoso de Oliveira
Santana, Márcio Antônio Portugal
Ribeiro, Isabela Gomes
Chaves, Daniel Gonçalves
Martins Filho, Olindo Assis
author_role author
author2 Santana, Márcio Antônio Portugal
Ribeiro, Isabela Gomes
Chaves, Daniel Gonçalves
Martins Filho, Olindo Assis
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silveira, Amanda Cardoso de Oliveira
Santana, Márcio Antônio Portugal
Ribeiro, Isabela Gomes
Chaves, Daniel Gonçalves
Martins Filho, Olindo Assis
dc.subject.en.none.fl_str_mv Immune regulation
Intracellular cytokine
staining Cytokine
profile FVIII
inhibitors Hemophilia A
topic Immune regulation
Intracellular cytokine
staining Cytokine
profile FVIII
inhibitors Hemophilia A
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-28T13:16:38Z
dc.date.available.fl_str_mv 2016-01-28T13:16:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVEIRA, Amanda Cardoso de Oliveira et al. The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors. BMC Hematology, vol. 15, n. 1, p. 1, 2015
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/12645
dc.identifier.issn.none.fl_str_mv 2052-1839
dc.identifier.doi.none.fl_str_mv 10.1016/j.molbiopara.2015.02.001
identifier_str_mv SILVEIRA, Amanda Cardoso de Oliveira et al. The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors. BMC Hematology, vol. 15, n. 1, p. 1, 2015
2052-1839
10.1016/j.molbiopara.2015.02.001
url https://arca.fiocruz.br/handle/icict/12645
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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