Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.

Bibliographic Details
Main Author: Gonçalves, Daniela da Silva
Publication Date: 2014
Other Authors: Cassimiro, Anna Paula Alvim, Oliveira, Caroline Dantas de, Rodrigues, Nilton Barnabé, Moreira, Luciano Andrade
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1186/1756-3305-7-228.
Download full: https://arca.fiocruz.br/handle/icict/9606
Summary: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
id CRUZ_00dcb4ea1bae975660d58abd49f721f0
oai_identifier_str oai:arca.fiocruz.br:icict/9606
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Gonçalves, Daniela da SilvaCassimiro, Anna Paula AlvimOliveira, Caroline Dantas deRodrigues, Nilton BarnabéMoreira, Luciano Andrade2015-03-04T13:00:51Z2015-03-04T13:00:51Z20141756-3305https://arca.fiocruz.br/handle/icict/960610.1186/1756-3305-7-228.engBioMed CentralWolbachia detection in insects through LAMP: loop mediated isothermal amplification.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilBACKGROUND: The bacterium Wolbachia is a promising agent for the biological control of vector-borne diseases as some strains have the ability to block the transmission of key human disease-causing pathogens. Fast, accurate and inexpensive methods of differentiating between infected and uninfected insects will be of critical importance as field-based trials of Wolbachia-based bio-control become increasingly common. FINDINGS: We have developed a specific and sensitive method of detecting Wolbachia based on the isothermal DNA amplification. This technique can be performed in an ordinary heat block without the need for gel-based visualisation, and is effective for a wide variety of insect hosts. CONCLUSION: Here we present the development of a rapid, highly sensitive and inexpensive method to detect Wolbachia in a variety of insect hosts, including key mosquito disease vectors.WolbachiaLAMP-loop mediated isothermal amplificationMosquitoInsectinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://arca.fiocruz.br/bitstreams/0db74c92-aee0-46be-8ace-c15e54800624/download7d48279ffeed55da8dfe2f8e81f3b81fMD51falseAnonymousREADORIGINAL2014_091.pdf2014_091.pdfapplication/pdf527173https://arca.fiocruz.br/bitstreams/5e1e6348-9d74-40f8-b2c6-cc135dcf6199/download7ebda7bf2dfb4a364f6783f1c65871caMD52trueAnonymousREADTEXT2014_091.pdf.txt2014_091.pdf.txtExtracted texttext/plain54168https://arca.fiocruz.br/bitstreams/cdad9afe-93c4-4255-9697-ac5cf507b569/download9d25466aa4acdc747308d0595413e299MD55falseAnonymousREADTHUMBNAIL2014_091.pdf.jpg2014_091.pdf.jpgGenerated Thumbnailimage/jpeg6176https://arca.fiocruz.br/bitstreams/8d71630c-95e5-4ab6-92d7-66eaa0c95b11/download17ead26281514aa85dd2fc6d2307514eMD56falseAnonymousREADicict/96062025-07-30 00:29:50.317open.accessoai:arca.fiocruz.br:icict/9606https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T03:29:50Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
dc.title.none.fl_str_mv Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
title Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
spellingShingle Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
Gonçalves, Daniela da Silva
Wolbachia
LAMP-loop mediated isothermal amplification
Mosquito
Insect
title_short Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
title_full Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
title_fullStr Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
title_full_unstemmed Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
title_sort Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.
author Gonçalves, Daniela da Silva
author_facet Gonçalves, Daniela da Silva
Cassimiro, Anna Paula Alvim
Oliveira, Caroline Dantas de
Rodrigues, Nilton Barnabé
Moreira, Luciano Andrade
author_role author
author2 Cassimiro, Anna Paula Alvim
Oliveira, Caroline Dantas de
Rodrigues, Nilton Barnabé
Moreira, Luciano Andrade
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Daniela da Silva
Cassimiro, Anna Paula Alvim
Oliveira, Caroline Dantas de
Rodrigues, Nilton Barnabé
Moreira, Luciano Andrade
dc.subject.en.none.fl_str_mv Wolbachia
LAMP-loop mediated isothermal amplification
Mosquito
Insect
topic Wolbachia
LAMP-loop mediated isothermal amplification
Mosquito
Insect
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-03-04T13:00:51Z
dc.date.available.fl_str_mv 2015-03-04T13:00:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/9606
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/1756-3305-7-228.
identifier_str_mv 1756-3305
10.1186/1756-3305-7-228.
url https://arca.fiocruz.br/handle/icict/9606
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://arca.fiocruz.br/bitstreams/0db74c92-aee0-46be-8ace-c15e54800624/download
https://arca.fiocruz.br/bitstreams/5e1e6348-9d74-40f8-b2c6-cc135dcf6199/download
https://arca.fiocruz.br/bitstreams/cdad9afe-93c4-4255-9697-ac5cf507b569/download
https://arca.fiocruz.br/bitstreams/8d71630c-95e5-4ab6-92d7-66eaa0c95b11/download
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
7ebda7bf2dfb4a364f6783f1c65871ca
9d25466aa4acdc747308d0595413e299
17ead26281514aa85dd2fc6d2307514e
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1839716297821126656

Similar Items