Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

Bibliographic Details
Main Author: Hassan,Mahmood ul
Publication Date: 2016
Other Authors: Akram,Zahid, Ali,Shaukat, Ali,Ghulam Muhammad, Zafar,Yusuf, Shah,Zahid Hussain, Alghabari,Fahad
Format: Article
Language: eng
Source: Crop Breeding and Applied Biotechnology
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200108
Summary: Abstract Peanut (Arachis hypogaea) is an important legume and oilseed crop, native to South America and grown in all tropical and temperate regions of the world. A simplified and rapid direct gene delivery system in peanut was developed by vortexing silicon carbide whiskers with callus and with plasmid harboring chitinase and hygromcin genes. The effects of callus age and whisker quantity on transformation efficiency were evaluated. Transformation efficiency (6.88%) was highest when 200 mg of whiskers were used with 5 µg plasmid for 2 g of 20-day-old callus. Hygromcin-resistant calli were regenerated to complete plants which produced seeds normally. Transgene insertion and number of transgene copieswere confirmed by PCR and southern blot analyses, respectively. Transgene expression was evaluated by a pathogenecity test and RT-PCR analysis. In transgenic events, the resistance level to leaf spot disease was far higher than in control plants.
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spelling Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot diseaseTransgeniccallusreporter genecell wallAbstract Peanut (Arachis hypogaea) is an important legume and oilseed crop, native to South America and grown in all tropical and temperate regions of the world. A simplified and rapid direct gene delivery system in peanut was developed by vortexing silicon carbide whiskers with callus and with plasmid harboring chitinase and hygromcin genes. The effects of callus age and whisker quantity on transformation efficiency were evaluated. Transformation efficiency (6.88%) was highest when 200 mg of whiskers were used with 5 µg plasmid for 2 g of 20-day-old callus. Hygromcin-resistant calli were regenerated to complete plants which produced seeds normally. Transgene insertion and number of transgene copieswere confirmed by PCR and southern blot analyses, respectively. Transgene expression was evaluated by a pathogenecity test and RT-PCR analysis. In transgenic events, the resistance level to leaf spot disease was far higher than in control plants.Crop Breeding and Applied Biotechnology2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200108Crop Breeding and Applied Biotechnology v.16 n.2 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n2a17info:eu-repo/semantics/openAccessHassan,Mahmood ulAkram,ZahidAli,ShaukatAli,Ghulam MuhammadZafar,YusufShah,Zahid HussainAlghabari,Fahadeng2016-08-01T00:00:00Zoai:scielo:S1984-70332016000200108Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2016-08-01T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
title Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
spellingShingle Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
Hassan,Mahmood ul
Transgenic
callus
reporter gene
cell wall
title_short Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
title_full Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
title_fullStr Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
title_full_unstemmed Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
title_sort Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
author Hassan,Mahmood ul
author_facet Hassan,Mahmood ul
Akram,Zahid
Ali,Shaukat
Ali,Ghulam Muhammad
Zafar,Yusuf
Shah,Zahid Hussain
Alghabari,Fahad
author_role author
author2 Akram,Zahid
Ali,Shaukat
Ali,Ghulam Muhammad
Zafar,Yusuf
Shah,Zahid Hussain
Alghabari,Fahad
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hassan,Mahmood ul
Akram,Zahid
Ali,Shaukat
Ali,Ghulam Muhammad
Zafar,Yusuf
Shah,Zahid Hussain
Alghabari,Fahad
dc.subject.por.fl_str_mv Transgenic
callus
reporter gene
cell wall
topic Transgenic
callus
reporter gene
cell wall
description Abstract Peanut (Arachis hypogaea) is an important legume and oilseed crop, native to South America and grown in all tropical and temperate regions of the world. A simplified and rapid direct gene delivery system in peanut was developed by vortexing silicon carbide whiskers with callus and with plasmid harboring chitinase and hygromcin genes. The effects of callus age and whisker quantity on transformation efficiency were evaluated. Transformation efficiency (6.88%) was highest when 200 mg of whiskers were used with 5 µg plasmid for 2 g of 20-day-old callus. Hygromcin-resistant calli were regenerated to complete plants which produced seeds normally. Transgene insertion and number of transgene copieswere confirmed by PCR and southern blot analyses, respectively. Transgene expression was evaluated by a pathogenecity test and RT-PCR analysis. In transgenic events, the resistance level to leaf spot disease was far higher than in control plants.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200108
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200108
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n2a17
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.2 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
_version_ 1754209187116613632

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