Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

• Main Author: Chen,Fangyu
• Publication Date: 2019
• Other Authors: Jiang,Liangrong, Huang,Rongyu, Chen,Xiaolong, Chen,Zhiming, Wang,Houcong, Huang,Yumin, Zheng,Jingsheng
• Format: Article
• Language: eng
• Source: Crop Breeding and Applied Biotechnology
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100110
• topic: Protein polymorphism; rice; leaf sheath; root; proteomics

Abstract To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F1), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.