Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration

Detalhes bibliográficos
Autor(a) principal: Chen,Lingdi
Data de Publicação: 2019
Outros Autores: Zhang,Yu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302019000200141
Resumo: SUMMARY Mirabegron is a kind of β3 adrenergic receptor agonist which is an effective drug for the treatment of overactive bladder. In this research, a UPLC–MS/MS method is developed and validated for the study of mirabegron pharmacokinetic in rats. A protein precipitation method is applied for sample preparation with acetonitrile. m/z 397.3→379.6, m/z 326.4→121.0 for mirabegron, tolterodine (IS), respectively in the positive ion mode was performed for quantitation. The method is reliable and reproducible in our study (intra-day precision≤11.06%, inter-day precision≤11.43%) with concentration curves linear from 5 to 2500 ng/mL(R2>0.999). Stability studies demonstrated that mirabegron was stable under a variety of storage conditions. This method was successfully applied for determining mirabegron in rats after oral and intravenous administration.
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spelling Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administrationUrinary bladder, overactiveAdrenergic beta-3 receptor agonistsPlasmaAdministration, intravenousAdministration, oralRatsSUMMARY Mirabegron is a kind of β3 adrenergic receptor agonist which is an effective drug for the treatment of overactive bladder. In this research, a UPLC–MS/MS method is developed and validated for the study of mirabegron pharmacokinetic in rats. A protein precipitation method is applied for sample preparation with acetonitrile. m/z 397.3→379.6, m/z 326.4→121.0 for mirabegron, tolterodine (IS), respectively in the positive ion mode was performed for quantitation. The method is reliable and reproducible in our study (intra-day precision≤11.06%, inter-day precision≤11.43%) with concentration curves linear from 5 to 2500 ng/mL(R2>0.999). Stability studies demonstrated that mirabegron was stable under a variety of storage conditions. This method was successfully applied for determining mirabegron in rats after oral and intravenous administration.Associação Médica Brasileira2019-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302019000200141Revista da Associação Médica Brasileira v.65 n.2 2019reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.65.2.141info:eu-repo/semantics/openAccessChen,LingdiZhang,Yueng2019-03-15T00:00:00Zoai:scielo:S0104-42302019000200141Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2019-03-15T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
title Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
spellingShingle Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
Chen,Lingdi
Urinary bladder, overactive
Adrenergic beta-3 receptor agonists
Plasma
Administration, intravenous
Administration, oral
Rats
title_short Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
title_full Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
title_fullStr Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
title_full_unstemmed Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
title_sort Determination of Mirabegron in rat plasma by UPLC–MS/MS after oral and intravenous administration
author Chen,Lingdi
author_facet Chen,Lingdi
Zhang,Yu
author_role author
author2 Zhang,Yu
author2_role author
dc.contributor.author.fl_str_mv Chen,Lingdi
Zhang,Yu
dc.subject.por.fl_str_mv Urinary bladder, overactive
Adrenergic beta-3 receptor agonists
Plasma
Administration, intravenous
Administration, oral
Rats
topic Urinary bladder, overactive
Adrenergic beta-3 receptor agonists
Plasma
Administration, intravenous
Administration, oral
Rats
description SUMMARY Mirabegron is a kind of β3 adrenergic receptor agonist which is an effective drug for the treatment of overactive bladder. In this research, a UPLC–MS/MS method is developed and validated for the study of mirabegron pharmacokinetic in rats. A protein precipitation method is applied for sample preparation with acetonitrile. m/z 397.3→379.6, m/z 326.4→121.0 for mirabegron, tolterodine (IS), respectively in the positive ion mode was performed for quantitation. The method is reliable and reproducible in our study (intra-day precision≤11.06%, inter-day precision≤11.43%) with concentration curves linear from 5 to 2500 ng/mL(R2>0.999). Stability studies demonstrated that mirabegron was stable under a variety of storage conditions. This method was successfully applied for determining mirabegron in rats after oral and intravenous administration.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302019000200141
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302019000200141
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9282.65.2.141
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.65 n.2 2019
reponame:Revista da Associação Médica Brasileira (Online)
instname:Associação Médica Brasileira (AMB)
instacron:AMB
instname_str Associação Médica Brasileira (AMB)
instacron_str AMB
institution AMB
reponame_str Revista da Associação Médica Brasileira (Online)
collection Revista da Associação Médica Brasileira (Online)
repository.name.fl_str_mv Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)
repository.mail.fl_str_mv ||ramb@amb.org.br
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