Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick

Detalhes bibliográficos
Autor(a) principal: Calaza,K.C.
Data de Publicação: 2000
Outros Autores: Gardino,P.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900014
Resumo: In this study we characterize the presence of muscarinic acetylcholine receptors (mAChR) in the isthmo-optic nucleus (ION) of chicks by immunohistochemistry with the M35 antibody. Some M35-immunoreactive fibers were observed emerging from the retinal optic nerve insertion, suggesting that they could be centrifugal fibers. Indeed, intraocular injections of cholera toxin B (CTb), a retrograde tracer, and double-labeling with M35 and CTb in the ION confirmed this hypothesis. The presence of M35-immunoreactive cells and the possible mAChR expression in ION and ectopic neuron cells in the chick brain strongly suggest the existence of such a cholinergic system in this nucleus and that acetylcholine release from amacrine cells may mediate interactions between retinal cells and ION terminals.
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spelling Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chickmuscarinic receptorsretinaisthmo-optic nucleuscentrifugal fibersacetylcholinechickIn this study we characterize the presence of muscarinic acetylcholine receptors (mAChR) in the isthmo-optic nucleus (ION) of chicks by immunohistochemistry with the M35 antibody. Some M35-immunoreactive fibers were observed emerging from the retinal optic nerve insertion, suggesting that they could be centrifugal fibers. Indeed, intraocular injections of cholera toxin B (CTb), a retrograde tracer, and double-labeling with M35 and CTb in the ION confirmed this hypothesis. The presence of M35-immunoreactive cells and the possible mAChR expression in ION and ectopic neuron cells in the chick brain strongly suggest the existence of such a cholinergic system in this nucleus and that acetylcholine release from amacrine cells may mediate interactions between retinal cells and ION terminals.Associação Brasileira de Divulgação Científica2000-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900014Brazilian Journal of Medical and Biological Research v.33 n.9 2000reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2000000900014info:eu-repo/semantics/openAccessCalaza,K.C.Gardino,P.F.eng2000-09-01T00:00:00Zoai:scielo:S0100-879X2000000900014Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2000-09-01T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
title Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
spellingShingle Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
Calaza,K.C.
muscarinic receptors
retina
isthmo-optic nucleus
centrifugal fibers
acetylcholine
chick
title_short Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
title_full Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
title_fullStr Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
title_full_unstemmed Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
title_sort Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
author Calaza,K.C.
author_facet Calaza,K.C.
Gardino,P.F.
author_role author
author2 Gardino,P.F.
author2_role author
dc.contributor.author.fl_str_mv Calaza,K.C.
Gardino,P.F.
dc.subject.por.fl_str_mv muscarinic receptors
retina
isthmo-optic nucleus
centrifugal fibers
acetylcholine
chick
topic muscarinic receptors
retina
isthmo-optic nucleus
centrifugal fibers
acetylcholine
chick
description In this study we characterize the presence of muscarinic acetylcholine receptors (mAChR) in the isthmo-optic nucleus (ION) of chicks by immunohistochemistry with the M35 antibody. Some M35-immunoreactive fibers were observed emerging from the retinal optic nerve insertion, suggesting that they could be centrifugal fibers. Indeed, intraocular injections of cholera toxin B (CTb), a retrograde tracer, and double-labeling with M35 and CTb in the ION confirmed this hypothesis. The presence of M35-immunoreactive cells and the possible mAChR expression in ION and ectopic neuron cells in the chick brain strongly suggest the existence of such a cholinergic system in this nucleus and that acetylcholine release from amacrine cells may mediate interactions between retinal cells and ION terminals.
publishDate 2000
dc.date.none.fl_str_mv 2000-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2000000900014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.33 n.9 2000
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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