Antimicrobial activity and physicochemical properties of different pastes used as intracanal medication

• Ano de defesa: 2016
• Autor(a) principal: Zancan, Rafaela Fernandes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
• Palavras-chave em Português: Ação Antimicrobiana; Antimicrobial action; Calcium Hydroxide; Hidróxido de Cálcio; Solubilidade; Solubility
• Link de acesso: https://www.teses.usp.br/teses/disponiveis/25/25147/tde-22102021-154824/

Introduction: The main function of intracanal dressing, in the treatment of teeth with pulpal necrosis, is the antimicrobial action against microorganisms which survived to the biomechanical preparation of root canal system. The combination of Calcium Hydroxide paste with other drugs has been suggested in order to increase the antiseptic capacity of the medication. The aim of this study was to evaluate the pH, calcium release, solubility and antimicrobial action on biofilms of the pastes: Calcium Hydroxide + Saline Solution (G1), Calen (G2), Calen CMCP (G3), Calcium Hydroxide + Chlorhexidine (G4), Double Antibiotic Paste (G5) and Triple Antibiotic Paste (G6). Material and methods: Measurement of pH and calcium release: acrylic teeth had their canal filled with the pastes in study (N=10) and the crown access sealed. Next, they were immersed in deionized water and after 3, 7, 15 and 30 days, removed from the flasks and put in a new flask. The pH and calcium ion of the water were measured by a pH meter and by an atomic absorption spectrophotometry, respectively. Solubility test: To evaluate the solubility, 60 acrylic teeth with standardized foramens (N=10) were filled with pastes and scanned at initial, 7, 15 and 30 days periods, before and after immersion in ultrapure water. The solubility of each specimen was the difference between the initial and final volume scanning. Antimicrobial activity: Biofilm in vitro of mono-specie (Enterecoccus faecalis) and dual-specie (Enterococcus faecalis + Pseudomonas Aeruginosa), were induced on blocks of bovine teeth. (N=20). Next, the samples were treated by the pastes for 7 days. The percentage of living cells were measured by using Live/Dead dye and confocal microscope. The data were statistically compared. Results: G1, G2, G3 and G4 did not present statistical difference to pH, calcium release and solubility values. G5 e G6 had the higher values of solubility. G4 e G6 presented the better action against biofilms. Conclusion:.The vehicle of paste seems not to interfere with pH, calcium release and solubility of calcium hydroxide pastes. None of the pastes killed 100% of the bacteria into the biofilm. Even with a high pH, 7 days may be an insufficient time for CH/P and CH/CMCP pastes to kill bacterial cells into the biofilm. Calcium hydroxide in addition to DAP, not favored its antimicrobial action. The TAP paste presented the bigger solubility and antimicrobial action.