The Circadian Oscillator and Thermopriming Interaction in the Heat Stress Response in Arabidopsis thaliana

• Ano de defesa: 2022
• Autor(a) principal: Matsukura, Bruno Fernandes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
• Palavras-chave em Português: Arabidopsis; Biological clock; Circadian oscillator; Estresse de calor; Heat-stress; Oscilador circadiano; Relógio biológico; Termocondicionamento, Termomemória; Thermomemory; Thermopriming
• Link de acesso: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-29112022-091005/

Heat stress is one of the critical problems faced by many crops. Heat waves are likely to become more frequent in the following years due to climate changes, affecting food production and nutritional values. Two mechanisms present in plants allow them to predict and prepare for upcoming events: priming and the circadian oscillator, or circadian clock. The first allows organisms to respond better to a stress condition if they have been previously exposed to a milder stress. The second allows organisms to prepare for diurnal and seasonal rhythmic events. The aim of this dissertation was to investigate how these two mechanisms are associated with the response to heat stress by comparing the thermoresistance and the gene expression between primed and non-primed Arabidopsis seedlings at different times of the day. We showed that both the circadian oscillator and the photoperiod influence thermopriming in Arabidopsis, affecting the acquired thermotolerance. In turn, thermopriming is capable of altering the expression of circadian oscillator components. Additionally, we observed that distinct sets of components appear to be involved in each time of the day thermopriming was more effective.