Influence of the addition of TiO2 nanotubes on the biocompatibility of Y-TZP ceramics and resin-based materials

• Ano de defesa: 2017
• Autor(a) principal: Lucena, Fernanda Sandes de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
• Palavras-chave em Português: Adesivo; Adhesive; Biocompatibilidade; Biocompatibility; Cimento Resinoso; Nanotubos de TiO2; Resin Cement; TiO2 Nanotubes; Zirconia; Zircônia
• Link de acesso: https://www.teses.usp.br/teses/disponiveis/25/25148/tde-30112021-092421/

The aim of this in vitro study was to evaluate the biocompatibility of yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP), a resin-based cement (RelyX Ultimate) and a 10- MDP-based adhesive (Single Bond Universal) modified or not by titanium nanotubes (TiO2), by means of the MTT cell viability test and Crystal Violet. For this purpose, disks of 13 mm in diameter per 2 mm is thickness of pre-sintered Y-TZP zirconia (IPS e.max ZirCAD) were obtained. The resin-cement and a 10-MDP adhesive disks were obtained through a metal mold with the same dimensions. For Y-TZP, the incorporation of TiO2 nanotubes occured before sinterization, while for the resin-based materials 0.3wt% of nanotubes were added to the uncured materials. The specimens were divided into 8 groups (n = 8). The in vitro evaluation was carried out by means of tests in which fibroblast line NIH 3T3 cells were placed into indirect contact with these materials. For cell viability were made MTT assay tests and Crystal Violet in duplicate and after 24, 48 and 72 hours the absorbance levels were analyzed by spectrophotometry Elisa reader. The data obtained were submitted to two-way ANOVA, followed by Tukey test ( = 0.05). In the period of 72 the highest increases of absorbance happened for the groups Y-TZP without TiO2 nanotubes and adhesive with TiO2 nanotubes when compared to the other groups. In general, the incorporation of nanotubes into these materials did not interfere with cell viability in both tests.