Acquired pellicle engineering with the association of Cystatin and Vitamin E against dental erosion

• Ano de defesa: 2023
• Autor(a) principal: Oliveira, Angélica Aparecida de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
• Palavras-chave em Português: Acquired enamel pellicle; Cistatina; Cystatin; Dental erosion; Erosão dentária; Modificação da película; Película adquirida do esmalte; Pellicle modification; Vitamin E; Vitamina E
• Link de acesso: https://www.teses.usp.br/teses/disponiveis/25/25145/tde-10112023-161625/

This study investigated the association of recombinant sugarcane cystatin (CaneCPI-5) and vitamin E in acquired enamel pellicle (AEP) engineering for the prevention of dental erosion in vitro. For such, two factors were studied: moment of AEP formation (before or after treatments) and type of treatment. For this purpose, 180 specimens of human enamel were divided into 12 groups: G1 and G7- CaneCPI-5 + Vitamin E; G2 and G8- Vitamin E + CaneCPI-5; G3 and G9- Vitamin E; G4 and G10-CaneCPI-5; G5 and G11 - Elmex (SnCl2/NaF/AmF); G6 and G12- Deionized water. Specimens from groups G1 to G6 were initially subjected to treatment (200 l; 2 min; 37 °C) and subsequently incubated to human saliva (200 l; 1 h; at 37 °C) to form the AEP. For groups G7 to G12, the AEP was formed first (200 l; 1 h; at 37 °C), and then the surface treatment was performed (200 l; 2 min; 37 °C). Erosive challenge was performed 3 times for all groups by immersion in critical acid (1%; pH 3.6; 1.0 ml; 1 min; 25 °C). Percentage of superficial hardness loss and relative surface reflection intensity (%SRI) were measured and analyzed by two-way ANOVA followed by Tukeys Test and Pearsons correlation (p < 0.05). In comparison to the other groups, the negative controls exhibited significantly lower protection (with a higher percentage of hardness loss and lower reflectivity) for both variables. Groups G1, G7 and G8 presented the lowest percentage of surface hardness loss. Groups G3 and G9 did not differ from group G2 but were different from the other groups (p<0.05). The greatest %SRI was found for the G1, G2, G3, G7, G8 and G9 groups, which did not difer signifcantly. The groups G4 and G5, did not difer signifcantly from the groups G10 and G11. There was a signifcant correlation between both analyses. The association of CaneCPI-5 and Vitamin E demonstrated showed a superior preventive effect than a commercial mouthwash stannous containing solution (Elmex® Erosion Protection) against initial erosion and can be considered a promising alternative for the formulation of solutions that aim to prevent erosive tooth wear.