Correlação entre os polimorfismos (G482A) e (G216A) do gene COMT (Catecol-o-Metiltransferase) e o ambiente no câncer de mama em cadelas

• Ano de defesa: 2018
• Autor(a) principal: Souza, Freddi Bardela de
• Orientador(a): Payão, Spencer Luiz Marques
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Sagrado Coração
• Programa de Pós-Graduação: Ciência e Tecnologia Ambiental
• Departamento: Ciências Exatas e Sociais Aplicadas
• País: Brasil
• Palavras-chave em Português: Polimorfismos; Câncer de mama; Cães
• Palavras-chave em Inglês: Polymorphism; Breast cancer; Dogs
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA
• Link de acesso: http://tede2.usc.br:8080/jspui/handle/tede/428

The number of cases of mammary tumors shows a marked increase in both animals and humans. The exact cause of this neoplasm is probably multifactorial. Hormonal influences, involvement of pro-inflammatory cytokines/chemokines, genetic polymorphisms and influences of environmental factors may be present. The aim of this study was to classify mammary tumors according to the World Health Organization (WHO), to evaluate the presence of polymorphisms (G482A) and (G216A) in the Catechol-O-Methyltransferase-COMT gene and to correlate them with the histological classification of as well as to study the correlation of the polymorphisms with some life habits of the animals and the places where they live. We evaluated 09 controls and 30 bitches with breast tumors, submitted to surgery at the Veterinary Hospital of FaculdadesIntegradas de Ourinhos - FIO and in the clinics of the region. In total, 39 samples were analyzed. The owners of the animals treated at the Veterinary Hospital of the Yarns answered a questionnaire of epidemiological character with the intention of associating some variables. In combination with the biopsy collection, blood was collected for DNA extraction and subsequent characterization of the COMT gene polymorphisms (G482A) and (G216A) by PCR-RFLP. The most common breast neoplasms were solid carcinomas and simple carcinomas, which were the most aggressive neoplasms according to the classification of the World Health Organization. Although the results were not statistically significant, a considerable association of Gene COMT in the 482A and 216A polymorphisms with the development of mammary neoplasiascan be observed, especially when the G allele with the G/G genotype was found.