Avaliação microbiológica das mãos de cirurgiões dentistas em atendimentos clínicos

• Ano de defesa: 2013
• Autor(a) principal: Carmo, Márcia Rosental da Costa
• Orientador(a): Weckwerth, Paulo Henrique
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: IASCJ - Universidade Sagrado Coração
• Programa de Pós-Graduação: Biologia Oral
• Departamento: Ciências da Saúde e Biológicas
• País: BR
• Palavras-chave em Português: ODONTOLOGIA; CONTROLE DE INFECÇÃO; AGENTES ANTIMICROBIANOS; MICROBIOLOGIA; LAVAGEM DE MÃOS; RISCO POR AGENTES BIOLÓGICOS
• Palavras-chave em Inglês: CROSS-INFECTION CONTROL; ANTI-INFECTIVE AGENTS; MICROBIOLOGY; HANDWASHING; EXPOSURE TO BIOLOGICAL AGENTS
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://tede2.usc.br:8080/jspui/handle/tede/226

Nowadays, in odontology it is noticed that the prevention of the microorganisms dissemination during the clinical procedures has been precarious, what makes the professional vulnerable to a lot of infectious diseases. Stopping the infections has become a big challenge for everyone involved. And it is worthy to say that the hands are the main vector to microorganisms dissemination. Because of that, this study was developed from the biological material collected from the hands of 41 dentists, randomly selected. The professional washed his/her hands for around 30 seconds in a sterile plastic bag, filled with 250 mL of physiologic serum, in three distinct times: Time 1 (T1), hands without wearing gloves, before attending the patient; Time 2 (T2), wearing gloves, right after the patient treatment, when the glove was contaminated with the patients biological material; and Time 3 (T3), collected after removing the gloves. The material obtained was inoculated in culture mediums such as: Brain Heart Infusion Agar (BHI), Manitol Salt Agar (MSA), Eosin Methylene Blue (EMB) and Bile Esculin Agar (BEA). There were identified Staphylococcus aureus and not aureus strains, besides Gram-negative bacterias, that suggest deficient hygiene of the hands. It was made the antibiogram for the Gram-positive and Gram-negative bacterias, the result showed a high number of strains resistant to the most common used antibiotics. The microbiological count was higher in the T1 for all the culture mediums, suggesting that the act of washing the hands for 30 seconds decreases the microbiota resident in the hands. For the purpose of complement the research, it was applied a questionnaire about the routine of the hands hygiene, gloves using and cares adopted for the control of infections. So, it was verified na ineffective conduct against the biological risks and inadequate hands hygiene habits.