Caracterização do potencial regenerativo das células-tronco derivadas de polpa dentária humana no reimplante dentário tardio com tratamento do dente incisivo de ratos Wistar

• Ano de defesa: 2015
• Autor(a) principal: Bisol, Ângela
• Orientador(a): Payão, Spencer Luiz Marques
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: IASCJ - Universidade Sagrado Coração
• Programa de Pós-Graduação: Biologia Oral
• Departamento: Ciências da Saúde e Biológicas
• País: BR
• Palavras-chave em Português: AVULSÃO DENTÁRIA; CÉLULAS-TRONCO; REIMPLANTE DENTÁRIO; TERAPIA CELULAR
• Palavras-chave em Inglês: DENTAL AVULSION; STEM CELLS; DENTAL REIMPLANTATION; CELLULAR THERAPY
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://tede2.usc.br:8080/jspui/handle/tede/247

The success of tooth replantation is limited due to root resorption that leads to loss of the replanted tooth. Progress in research on stem cells represent new prospects for regeneration of lost dental tissues. Stem cells are capable of selfrenewal and differentiation into multiple cell lineages, this process called plasticity ensures the maintenance of a stock of undifferentiated cells capable of repair or renovation of new tissue. Stem cells are a focus of dental research. This research aims to evaluate the influence of stem cells from the pulp of human exfoliated deciduous teeth on the supporting tissues of the thooth. Mesenchymal stem cells from the pulp of human deciduous teeth were applied associated to a scaffold of platelet rich plasma (PRP), to the dental alveolus of rats at the time of dental reimplantation, sixty minutes after extraction, with endodontic treatment and root surface treatment as the protocol. Fifty-four rats (Rattus norvegicus albinus, Wistar) underwent a surgical procedure for extraction and reimplantation of the right upper incisor. The animals were divided into 3 experimental groups. In group I, the control group, the incisor was replanted without any substance; in group II, the tooth was reimplanted with stem cells associated with platelet-rich plasma and in group III - incisor was replanted with only platelet-rich plasma in the tooth socket. The animals were observed for periods of 15, 30 and 60 days. Animals were euthanized for analysis by histological techniques and histomorphometrical analysis. The results showed the occurrence of replacement resorption and ankylosis in the three groups, thus in the stem cells group teeth were more preserved but with no statistically significant difference between groups (p>0.05)