Avaliação da concentração inibitória e bactericida mínima de dois cimentos tipo portland puros e acrescidos de antibióticos

• Ano de defesa: 2013
• Autor(a) principal: Giraldi Neto, Francisco Orlando
• Orientador(a): Weckwerth, Paulo Henrique
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: IASCJ - Universidade Sagrado Coração
• Programa de Pós-Graduação: Implantologia
• Departamento: Ciências da Saúde e Biológicas
• País: BR
• Palavras-chave em Português: CIMENTO PORTLAND; ATIVIDADE ANTIMICROBIANA; AMOXICILINA; CIPROFLOXACINA; DILUIÇÃO EM CALDO
• Palavras-chave em Inglês: PORTLAND CEMENT; ANTIMICROBIAL ACTIVITY; AMOXICILLIN; CIPROFLOXACIN; TUBE DILUTION TEST
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://tede2.usc.br:8080/jspui/handle/tede/230

The aim of this study was to evaluate the in vitro antimicrobial effects of pure White Portland cement and zirconium oxide plus in the ratio of 1:1 (50% white Portland cement and 50% zirconium oxide), added amoxicillin and ciprofloxacin after solidification and also determine the minimum inhibitory concentration and minimum lethal concentration of these products. After adding up to 10% of cement weight of amoxycillin and ciprofloxacin, separately, they were spatulate with distilled water, awaiting prey 24 hours at 37 ° C. The material was crushed manually in degree and pestle until the consistency of fine powder and smooth. One hundred and twenty vials were prepared containing BHI and cements were inserted directly into the tubes at concentrations of 5 mg / ml, 2.5 mg / ml, 1.25 mg / ml, 0.625 mg / ml and 0.313 mg / ml and mechanically agitated for better dispersion. Strains from the American Type Culture Collection (ATCC) of Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922) and Enterococcus faecalis (ATCC 29212) were obtained. A fresh inoculum of each organism was prepared and seeded directly in the tubes, each tube in each subgroup received a microorganism at each concentration. The tubes were then incubated at 37 ° C and the turbidity was assayed at 24 and 48 hours. After 24 hours incubation at 37 ° C, all tubes of the groups Portland Portland 50% pure and were hazy, indicating growth microbiano.No subgroup Portland + amoxicillin MIC was only Escherichia coli concentration of 2.5 mg / ml and 5 mg / ml. In subgroup Portland 50% + amoxicillin MIC was only Escherichia coli at a concentration of 5 mg / ml. In all other concentrations, bacterial growth was evident. The subgroups + ciprofloxacin and Portland Portland 50% + ciprofloxacin, showed no bacterial growth at all concentrations. In all positive controls bacterial growth. We conclude that addition of ciprofloxacin to Portland cement favored inhibitory effect and bactericidal cement after trapped in vitro and amoxicillin, at the concentration of 2.5 mg / ml for Portland pure and 5 mg / ml for Portland 50%, favoring the effect inhibitory for E. coli.