Detalhes bibliográficos
| Ano de defesa: |
2011 |
| Autor(a) principal: |
SILVA, Kaliny Veiga Pessoa da
 |
| Orientador(a): |
CARVALHO, Reginaldo de |
| Banca de defesa: |
MELO FILHO, Péricles de Albuquerque,
MELO, Maria Betânia de Oliveira,
FRAZÃO, Jailson Gitaí dos Santos,
CAMARA, Terezinha de Jesus Rangel |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Melhoramento Genético de Plantas
|
| Departamento: |
Departamento de Agronomia
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6446
|
Resumo: |
The Manihot genus belongs to Euphorbiaceae family, has about 98 species and native to tropical regions of the Americas, with greatest diversity center in Brazil, with 80% of Manihot species, showing a large vegetative polymorphism and a potential source for cassava breeding programs. Cassava (Manihot esculenta Crantz) is the only commercially cultivated species, with the shoots and the tuber roots used for both human food and animal feed. Cassava roots are also used in the manufacture of flour or in the composition of other products. Karyotypic analysis in mitotic or meiotic cells concerning to chromosomal homology, numerical and structural variations, polyploidy and evolution mechanisms of the karyotypes can provide useful information for breeding programs aimed at achieving improved cultivars. In addition, a karyotype study in many cases contributes to the increase cytogenetic markers that while certain aspects related to horticultural assist in the cultivars characterization. Manihot species are considered allotetraploid, with 2n=36 chromosome and x=9 as basic number. Natural interspecific crosses can be found frequently, making in some cases infertile hybrids. Infertility is not easily detected using phenotypic analysis. However, it is believed that these species undergone diploidization process along the evolution, now showing a meiotic behavior of a diploid. This work aimed the mitotic and meiotic analysis in nine species of the genus Manihot in order to confirm the karyotypic stability described at literature data. Three varieties of cassava and eight wild species were analised. The analysis revealed strong mitotic stability among species regarding the number and chromosome morphology, average size of chromosomes of 1.75 and maximum of two pairs of satellites. The meiosis was regular in wild species and irregular in varieties of M. esculenta 'manipeba', showing univalent, bivalent and trivalent at metaphase-anaphase I, showing typical behavior of a triploid and partly irregular meiosis in 'pornunça', producing polyads in microsporogenesis. An additional study was performed with molecular marker ISSR (Inter simple sequence repeat). Polymorphism was observed in 89.7% among the locus of the species, but as expected, there was a great genetic similarity between varieties of M. esculenta cultivated for the wild species. |
