Potencial patogênico de Escherichia coli multirresistentes a antibióticos isoladas de carcaças de frango aprovadas para o consumo humano no Estado de Pernambuco, Brasil

• Ano de defesa: 2014
• Autor(a) principal: VAZ, Renata Valença
• Orientador(a): LIMA FILHO, José Vitor Moreira
• Banca de defesa: OLIVEIRA, Jaqueline Bianque de, RABELLO, Carlos Bôa Viagem, CAVALCANTI, Yone Vila Nova
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal Rural de Pernambuco
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciência Animal Tropical
• Departamento: Departamento de Morfologia e Fisiologia Animal
• País: Brasil
• Palavras-chave em Português: Potencial patogênico; Escherichia coli; Carcaça de frango
• Palavras-chave em Inglês: Pathogenic potential; Carcass chicken
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4641

In the present study, we investigated the pathogenic potential of Escherichia coli isolates from samples of chicken livers from carcasses approved for human consumption. The samples ( n = 110 ) were obtained from an abattoir in the State of Pernambuco , Brazil. The bacterial isolates were presumptively identified form Agar Eosin Methylene Blue. The antibiotic resistance profile of the isolates was assessed by the disk diffusion method, according to criteria established by the Committee for Clinical Laboratory Standards Institute (CLSI). Hereafter, ten isolates resistant to three or more antibiotics and ten susceptible isolates were selected and tested for resistance to serum of chicken and human tests. Our results demonstrated the existence of isolates with resistance phenotypes to streptomycin (84.04 %) , tetracycline (44.68 %) , Amikacin (29.78% ) Ceftazidina (21.27 %) and gentamicin (21.27%) . Likewise, in general , the multiresistant isolates showed resistance to the bactericidal effects of serum and human serum birds. The multiresistant isolates (n = 20 ) were phylogenetically investigated and screened for the presence of ISS gene (Increased serum survival). It was observed that the strains were distributed between the four main phylogenetical groups (B2, D, B1, A) and seven isolates of groups B2, B1 and D had the gene iss . In conclusion, our results indicate the presence of E. coli strains multiresistant to antibiotics and potentially pathogenic in chicken carcasses approved for human consumption.