Detalhes bibliográficos
| Ano de defesa: |
2010 |
| Autor(a) principal: |
LAPENDA, Anízia Maria Vieira de Souza
 |
| Orientador(a): |
CARVALHO NETO, Pedro Marinho de |
| Banca de defesa: |
STAMFORD, Tânia Lúcia Montenegro,
MOTA, Rinaldo Aparecido |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
|
| Departamento: |
Departamento de Ciências Domésticas
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5071
|
Resumo: |
Because Listeria spp. especially L. monocytogenes, a major concern of food industry and public health agencies, the ability to survive and multiply under conditions technically suitable for food preservation as coolant temperature, coupled with its resistance to freezing, heat and various antibiotics, became microorganism emerging and very important among the pathogens transmitted by foods such as meat sausages, raw and cooked poultry, raw meat, raw and smoked fish ready for consumption. This study aimed to evaluate the occurrence of Listeria spp. in sausage samples cooled. We analyzed 48 samples of hams and sausages, obtained from public markets and supermarkets in six regions distributed Political Administration of the city of Recife, the presence and absence of Listeria spp. through enzyme immunoassay and conventional methods, and to evaluate the profile of antimicrobial sensitivity by disk diffusion method. Regarding the presence of Listeria spp. stood out the VIDAS® LDUO system for detecting 28 (58.33%) samples positive for Listeria spp., whereas the conventional method were detected 27 (56.25%) positive samples, of which 15 (53.57% ) samples were characterized for L. monocytogenes. Thus revealing a high percentage of Listeria monocytogenes in food analysis and giving the method a high sensitivity and specificity for the agent studied. The profile of antimicrobial sensitivity of the samples tested showed values between 92.6% to 100% for the antibiotics tested, leaving only the profile of ciprofloxacin with a sensitivity of 78%, also emphasizing the presence of a sample of Listeria monocytogenes multiresistant to Vancomycin, Ampicillin, Amoxicillin, and Ciprofloxacin. The results do not explain the origin of this contamination and thus require, monitor the steps of producing such food industries and retail trade and establish limits for L. monocytogenes in meat foods chilled ready to eat in order to enable a security in the supply of quality food. |
