Detalhes bibliográficos
| Ano de defesa: |
2010 |
| Autor(a) principal: |
FREITAS, Soraya Farias de Andrade
 |
| Orientador(a): |
BARBOSA, Severino Benone Paes |
| Banca de defesa: |
GOMES FILHO, Manoel Adrião,
SANTORO, Clebe Régis,
MONARDES, Humberto Gonzalo |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Zootecnia
|
| Departamento: |
Departamento de Zootecnia
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6918
|
Resumo: |
The aim of the present work was to detect the genetic polymorphism of the β-Lactoglobulin (β-Lg) in different groups of Holstein-Gir breed cattle. There was collected 165 animals’ blood samples from the experimental farms of the Instituto Agronômico de Pernambuco (Itambé e Arcoverde) and from a private farm located in Zona da Mata Sul in the state of Pernambuco. The genetic groups were 1/2, 3/4, 5/8 Holstein and 5/8 H 1st, 2nd, 3rd and 4th bi-crossbred generation. For the identification of genotypes, a located fragment was amplified in the areas of exons II and III of β-Lg gene using the PCR technique. The result of the amplification was submitted to the PCR-RFLP analysis to digest the fragment by HaeIII endonuclease and it produced fragments of 400, 220, 190, and 151pb molecular weight for all animals, equivalent to the heterozygous genotype (100% AB) of β-Lg. The Pearson's chi-square test (2)and considering the observed and expected genotypic frequencies of the genotypes β-Lg. Was verified that frequences differed significantly at the level tested (P<0,05), indicating that the samples studied was not in Hardy-Weinberg equilibrium. |
