Sexagem fetal em líquido amniótico ovino (Ovis aries L.,1758) por PCR

Detalhes bibliográficos
Ano de defesa: 2008
Autor(a) principal: MELO, Arthur Nascimento de lattes
Orientador(a): WISCHRAL, Aurea
Banca de defesa: SILVA, MÁrcia Bezerra da, PORTO, Ana Lúcia Figueiredo, GOMES FILHO, Manoel Adrião
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural de Pernambuco
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciência Veterinária
Departamento: Departamento de Medicina Veterinária
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5270
Resumo: In order to achieve the identification of sex in fetal sheep, were subjected to the technique of Polymerase Chain Reaction (PCR), 45 samples of DNA extracted from amniotic fluid of pregnant uterus, 24 samples of males and 21 females. The amniotic fluid was obtained by amniocentesis guided by ultrasound for greater accuracy in aspiration and also for subsequent application of the technique in vivo, as simulation. Gestational age was laid on the measurement of fetuses with caliper, which showed variation of 3.3 to 46.5 cm. The samples were divided into three groups based in Kadu and Kaikini (1987): Group 1 (zero to 8.6 cm) from conception to the 60th day of gestation, group 2 (8.7 to 26 cm) among 61th and 105th day, and Group 3 (26,1 cm on) 106th day until the birth. The DNA was extracted using the method phenol-chloroform, obtaining a quantity of less than 50 ng per μL, quantified by comparing through - lambda and visualized in 1% agarose gel. For amplification of genetic material, were used "primers" to the SRY gene (116pb), found in the Y chromosome present only in males, and Aml-X (300pb), autosomal, present in both sexes. In all samples was possible to sex the fetuses and the primers used shown to be quite effective. The sex was confirmed by morphological analysis of fetuses, aside from the concept who genital tubercle still had not moved, proving the effectiveness of the PCR molecular tool for this examination.