Epidemiologia clássica e molecular da tuberculose pulmonar em pacientes da região norte de Minas Gerais

• Ano de defesa: 2013
• Autor(a) principal: Prince, Karina Andrade de [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Tuberculose; Epidemiologia molecular; Pulmões - Doenças; Mycobacterium tuberculosis; Tuberculosis - Diagnosis
• Link de acesso: http://hdl.handle.net/11449/108419

Tuberculosis is a chronic infectious disease caused by the bacillus aerobic Mycobacterium tuberculosis. It causes health problems among millions of people each year, being ranked as the second leading cause of death from infectious disease worldwide. Thus, the aim of this study was to analyze the epidemiology of classical and molecular pulmonary tuberculosis in patients in North of Minas Gerais. We conducted an epidemiological study, an observational, longitudinal, retrospective and quantitative design. The population consisted of patients with suspected pulmonary TB, residents in the north of Minas Gerais patients at the University Hospital Clemente de Faria (HUCF), State University of Montes Claros (UNIMONTES), from January 2011 to December 2012. The clinical samples (sputum or bronchoalveolar lavage) of these patients were analyzed by Ziehl-Neelsen staining and culture by the method of Ogawa-Kudoh. Clinical isolates were subjected to molecular identification, genotyping by Spoligotyping techniques, RDRio, ERIC-PCR and MIRU-VNTR and drug susceptibility testing. The classical and molecular epidemiological correlation was performed using data from genotyping and classical epidemiological data. Of the 345 patients evaluated, 33 (9.6%) had the disease, with 69.7 % living in Montes Claros and 30.3 % in other cities in northern Minas Gerais. There was a prevalence of TB in males (75.8%), of mulattoes (60.6%), low education (42.4 %) and working age (78.9%) . The majority of new cases were (69.7%) and the smear was positive in 75.8 % of patients. The cure rate was low (30.3%) and high transfer (30.3 %). The most obvious grievances remain, the co -infection TB / HIV (6.1%) and alcoholism (24.25%) . Analysis of 33 clinical isolates by Spoligotyping allowed the identification of 22 different genetic profiles (66.7% genetic diversity). Among the genetic profiles found 15 (45.5%) LAM, 04 (18.2 %) belong to T family, 01 families (3.0%) to Haarlen ...