Análises histopatológicas, bioquímicas e moleculares sobre os efeitos da interação entre o uso de altas doses de decanoato de nadrolona e o exercício resistido durante a fase pós-púbere sobre a próstata de ratos em processo de envelhecimento

• Ano de defesa: 2014
• Autor(a) principal: Gomes, Fabiana de Campos [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Decanoatos; Próstata - Inflamação; Exercicios fisicos - Aspectos fisiologicos; Citocinas; Receptores Toll-like
• Link de acesso: http://hdl.handle.net/11449/123786

Anabolic-androgenic steroids are used by professional and recreational athletes who want to increase muscle mass. The Nandrolone Decanoate (ND) is the most widely used steroid among athletes. Together, ND and physical resistance training (PRT) may alter the androgen levels and lead disturbances in the prostate homeostasis. The present study aimed to investigate the effects of the ND abusive use and PRT on adult and older rats prostate. We evaluated whether ND at doses of 5mg/kg (twice a week, via i.m.), with or without physical exercises, is able to alter the prostate morphophysiology. Materials and Methods: 56 male Sprague-Dawley rats were divided into four groups (n=7): sedentary and trained groups, with or without ND. The animals were treated for eight weeks and then sacrificed 48h after the last injection (adults) or sacrificed with 300 days old. The prostate was removed and processed to morphometric and histopathological analyses and according to Western blotting techniques for NOX1, NrF2, TLR2 and 4, NFκB, IRF3, MAPK, IL-6 and TNFα expressions. The results showed that both ND and PRT altered the testosterone levels and estradiol level just in DN groups. ERα and ERβ increased in animals that used DN in adulthood and ER β expression decreased in treated groups aged. The PRT was involved in AR reducing in aged animals. DN and PRT during adulthood lead to pathological lesions of medium to high grade and activated TLR4. TLR2, Nox1 and Nrf2 expression were increased DN groups. The pathological lesions induced NFκB, IRF3 and MAPK expression, with TNF and IL-6 production. Despite no alterations were observed in the expression of TLR2 and TLR4 in SD2 and EV2 groups, NFκB and cytokine expressions were increased. Thus, we conclude that ND, associated or not to PRT alter hormone levels, triggers an inflammatory pathway and interferes with the prostate morphophysiology during adulthood and aging