Efeito da curcumina na angiogênese em modelo experimental de câncer de mama

• Ano de defesa: 2014
• Autor(a) principal: Ferreira, Lívia Carvalho [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Genética; Cancer - Aspectos geneticos; Mamas - Cancer; Neovascularização; Proteínas angiogênicas; Curcumina; Genetics
• Link de acesso: http://hdl.handle.net/11449/111000

Breast cancer is the most common cancer in women being rated as the second most common in the world. Tumor growth requires the formation of new vessels that are stimulated by angiogenic factors and their receptors. The neoplastic cells are able to modify their phenotype to promote functional changes and stimulate the production of proteins involved in angiogenesis. Curcumin, a component of the extract of Curcuma longa L, is used both as food and in traditional medicine, however some evidence indicates that this extract has oncostatic effects in different types of cancer. The aim of this study was to evaluate the effects of treatment with curcumin in tumor progression and angiogenesis in an experimental model of breast cancer. The cell line of human breast cancer (MDA-MB-231) was cultured and the rate of cell viability was measured by MTT assay after treatment with different doses of curcumin. For in vivo study, the cells were implanted in female athymic nude mice, which were randomly divided into treated (n = 5) and control animals (n = 8). The animals received daily 300 mg/kg of curcumin or vehicle daily, starting in the same day as tumor implantation and continued for 21 days five times per week. Tumor size was measured weekly with a digital caliper. The end of treatment, angiogenesis was assessed in vivo by the technique of computed tomography single photon emission tomography (SPECT) with the radiotracer Tc-99m-HYNIC-VEGF-c protein coupled recombining, which has affinity for VEGFR2/3. Furthermore, the expression of VEGF-A, VEGF-C e VEGFR2/3 marker of cell proliferation (Ki-67) and von Willebrand factor (vWF) receptors were verified by immunohistochemical. Curcumin treatment in vitro was able to significantly decrease cell viability (p < 0.05) Animals treated with curcumin showed less tumor volume (232.5 ± 53.2 mm 3) compared to control animals (282.0 ± 88.5 mm3), however, no significant difference (p > 0,05). The radioactivity of ...