Abordagens biossintéticas em piper tuberculatum auxiliada pela síntese de precursores fenilpropanoídicos

• Ano de defesa: 2014
• Autor(a) principal: Pioli, Renan Moraes [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Quimica organica; Biossintese; Sintese; Produtos naturais; Natural products
• Link de acesso: http://hdl.handle.net/11449/110697

The increase in demand for discovery of new drugs from plant origin has led also to increase organic studies to elucidate the metabolic pathway of the secondary metabolites of interest. Often, these metabolites show low concentration in plant as well structural and configurational complexity distinguishes them from synthetic products and prohibit their synthesis or semi - synthesis. Thus, understanding the biosynthetic processes involved in their formation can facilitate to obtain them by biotechnology techniques. Therefore, the Piper tuberculatum species known to accumulate piperidinic, piperidonic and isobutyl amides that show potential antitumoral, acaricidal, antifungal and insecticidal activities, is set in an excellent model for such functional studies. From leaves and fruits extract two compounds were isolated, the piperine and piplartine, main amides found in Piper tuberculatum. The synthesis of phenylpropanoids precursors: 3'-4'- methylenedioxycynamic acid and ferulic acid as well the same compounds labeled with stable isotopes: acid [1 -OD , 2- D]-3'-4'- methylenedioxycynamic acid and [1 -OD , 2- D]- ferulic acid were performed for the biosynthetic studies. The precursors, L–lysine, 3'-4'- methylenedioxycynamic acid and malonic acid were incorporated in the soluble enzyme fraction of Piper tuberculatum. The optimum conditions for extraction of the enzyme were based on three parameters; temperature (31 ° C), time of reaction (90 minutes) and concentration of precursors (10 mM). The products were analyzed by liquid chromatography and mass spectrometry. The analyzes were monitored using piperine as standard and its formation was determined. For determination of protein concentration the Bradford method was used. The analyze of the proteins was performed by using one-dimensional electrophoresis (SDS- PAGE).