Exercício físico, ingestão de frutose e marcadores da síndrome metabólica em modelo experimental

• Ano de defesa: 2014
• Autor(a) principal: Botezelli, José Diego [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Physiology; Fisiologia; Exercícios físicos; Frutose; Síndrome metabólica; Disturbios do metabolismo; Obesidade; Marcadores biologicos; Stress oxidativo
• Link de acesso: http://hdl.handle.net/11449/108761

Metabolic Syndrome has become serious medical-social problem in developed and development country and the excessive fructose consumption in the occidental diet has a major role in this question. This way, it has been a great interest the development of procedures more effectives to prevent and treat this disease. The non-alcoholic fatty liver disease (NAFLD) emerges as a syndrome component and can be related to the development of insulin resistance. Once human studies have many limitations it is necessary the use of animal models adequate to this disease study. This research aims to investigate the potential effects of the physical exercise in the pathophysiology of fructose-induced metabolic syndrome, oxidative stress and inflammation. The first step aimed to analyses the effects of fructose-rich diet in metabolic syndrome markers. We used Wistar rats (120 days), separated in two groups, according to the diet: control (balanced Labina ® dieta) and fructose-rich diet (60% of caloric content provided by fructose). The animals fed on a fructose rich diet for 60 days (120-180 days). At the end of experiment, they were euthanized to determine in order: the glucose tolerance, insulin sensitivity, insulin levels, biomarkers of nonalcoholic fatty liver disease, biomarkers of oxidative stress and liver histology. The animals fed on fructose rich-diet showed lower glucose tolerance, insulin sensitivity, hiperinsulinemia, raises on serum glucose and triglycerides, higher AST/ALT ratio, fat accumulation in the liver and in the mesenteric adipose tissue, a reduction in the anti-oxidant system activities and higher levels of TBARs. The second step consisted to evaluate the effects of three exercise protocols in Wistar rats (120 days) on metabolic syndrome markers. Quarter of the animals were kept sedentary (C) during all the experiment (120-180 days); a quarter animals trained in the aerobic protocol (A) (80% of minimal lactate) 1h day, 5 days week,...