Efeito de soluções contendo hexametafosfato de sódio e quercetina, associados ou não ao fluoreto, sobre o desgaste erosivo dentinário in vitro

• Ano de defesa: 2022
• Autor(a) principal: Capalbo, Letícia Cabrera [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Erosão dentária; Fosfatos; Quercetina; Dentina; Inibidores de Metaloproteinases de Matriz; Tooth erosion
• Link de acesso: http://hdl.handle.net/11449/234559

The aim of the present study was to investigate the effect of solutions containing fluoride (F), sodium hexametaphosphate (HMP) and quercetin (QC), alone or in association, on dentin erosion and on the inhibition of matrix metalloproteinases (MMPs) - 2 and -9, using in vitro protocols. Bovine root dentin blocks (4 × 4 × 2 mm; n = 96), selected by surface hardness, were randomly divided into 8 experimental groups (n = 12/group) and treated 2×/day (one minute) with the following solutions: (1) deionized water (negative control); (2) 1100 ppm F (“F”); (3) 1.0% HMP (“HMP”); (4) 0.03% QC (“QC”); (5) F+HMP; (6) F+QC; (7) HMP+QC; and (8) F+HMP+QC. Blocks were submitted to erosive challenges 4×/day for 5 days (dynamic exposure to 50 mmol.l-1 citric acid, pH 3.2, 90 s). They were then analyzed for dentin loss (profilometry) and integrated hardness loss in depth (area under the curve, ΔKHN). The antiproteolytic potential of solutions containing F, HMP and/or QC was analyzed by zymography. Dentin loss results (log10 transformed) were submitted to one-way ANOVA, followed by Tukey's test. ΔKHN data (raw) were submitted to two-way, repeated-measures ANOVA, followed by the Holm-Sidak test (p<0.05). The lowest dentin erosive wear was promoted by F+HMP+QC. At the lowest depths (5-30 μm), blocks treated with F+HMP+QC showed the highest values of ΔKHN. Zymography analysis showed that all treatments completely inhibited MMP-2 activity, except for QC administered alone (77% inhibition). For MMP-9, all the solutions containing HMP or the association of F+QC promoted total antiproteolytic activity. It was concluded that the addition of HMP and QC to F solutions led to greater protection against dentin erosion, both at the surface (dentin loss) and in relation to the mineral content of the remaining tissue (ΔKHN), in addition to promoting a complete inhibition of MMPs -2 and -9 activity in vitro.