Avaliação proteômica do conteúdo vaginal em resposta ao tratamento da vaginose bacteriana

• Ano de defesa: 2015
• Autor(a) principal: Ferreira, Carolina Sanitá Tafner [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Aparelho genital feminino; Proteômica; Vaginose bacteriana; Bacterioses; Vagina - Infecções; Vaginosis, Bacterial
• Link de acesso: http://hdl.handle.net/11449/131919

Bacterial vaginosis is the most common type of abnormal vaginal flora and defined by the depletion of vaginal lactobacilli. This condition increases the risk of premature labor and acquisition of several sexually transmitted infections. Short-term efficacy of bacterial vaginosis metronidazole treatment is low. Thus, we aimed to characterize the cervicovaginal fluid proteome of women with bacterial vaginosis and to compare the proteomic profile between women who were successfully treated with those who failed to reestablish lactobacillar flora after the 7-days course of metronidazole. Presence of bacterial vaginosis was defined according to Nugent criteria on Gram-stained vaginal smears. Proteomic profile of cervicovaginal fluids were determined using shotgun LC MS/MS. Comparative analysis of the proteome of 38 women with bacterial vaginosis and 39 with normal vaginal flora identified and determined the relative abundance of 116 proteins. Among them, cathepsin G and Ig heavy chain V-III region BRO were exclusive of bacterial vaginosis while leukocyte elastase inhibitor, involucrin and neuroblast differentiation-associated protein AHNAK of normal flora. Moreover, 20 (17.2%) proteins were differentially expressed in bacterial vaginosis, of which 9 are involved in immune response. However, the cervicovaginal proteome of 24 women with bacterial vaginosis who were successfully treated and 11 who persisted with this condition did not differ between each other. Therefore, we showed that bacterial vaginosis significantly changes the local proteome, but cervicovaginal host's proteins do not influence the response to metronidazole treatment