Estudo da migração de células peritoneais na paracoccidioidomicose experimental murina: avalaição do efeito da estimulaçao de células peritoneais por lectina sobra a evolução da lesão paracoccidióidica

• Ano de defesa: 2015
• Autor(a) principal: Marchetti, Camila Martins [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Paracoccidioidomicose; Imunologia; Citologia; Resposta imune; Camundongo como animal de laboratorio; Paracoccidioidomycosis; Immune response
• Link de acesso: http://hdl.handle.net/11449/126369; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/27-07-2015/000841564.pdf

The paracoccidioidomycosis (PCM) is an infectious disease caused by species of the genus Paracoccidioides, an intracellular pathogen that depends on an effective and protective specific-host cellular immune response. In this context, the peritoneal environment emerges as a possible site for immunotherapy, since phagocytes exhibit plastic biological functions related to induce/regulate inflammation, angiogenesis and tissue remodeling processes as well as to migrate to inflamed and infected tissue. Recently, it was reported that peritoneal cells stimulated with Concanavalin A (Con-A) show an increased cell activation characterized by high phagocytic activity, increased production of TNF-α and expression of mannose receptor. Thus, this study aimed to identify a possible action of Con-A in the peritoneal cavity, specifically the migration of peritoneal phagocytes and the profile of the response by peritoneal phagocytes in a murine model of pulmonary paracoccidioidomycosis. To this, BALB/c mice were used, infected intratracheally with the fungus or no. Assessment of migration of peritoneal phagocytes was by vital dye PKH26. The treatment with Con-A was by intraperitoneal route, the doses were given with an interval of four days (peak of macrophage activity) and the peritoneal phagocytes were analyzed in vitro for production of IL-6, IL-10, TNF-α, MCP-1, IFN-γ e IL -12p70 and reactive oxygen species by. Our findings showed that the treatment with three doses of Con-A in no-infected mice induces migration of peritoneal cells to the lung and also a pro-Th1 profile by peritoneal phagocytes. Furthermore, the treatment with three doses of Con-A lead a protective immune response in mice infected with P. brasiliensis, characterized by increased production of H2O2 in the presence or absence of P. brasiliensis antigens and in the production of IFN-γ and NO in the presence of fungal antigen. Despite of these findings, no changes were observed in the ...