Efeitos da ciclosporina na lesão renal por isquemia-reperfusão em ratos: estudo em modelo experimental

• Ano de defesa: 2015
• Autor(a) principal: Oliveira, Antonio Carlos Cerqueira de [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Rins - Doenças; Ciclosporina; Isquemia; Insuficiencia renal aguda; Drogas - Efeito fisiologico; Rato como animal de laboratorio; Cyclosporine
• Link de acesso: http://hdl.handle.net/11449/131974; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/24-11-2015/000854498.pdf

Kidney ischemic injury contributes decisively to the increase of morbidity and mortality. Various strategies of intervention in Ischemia-Reperfusion Injury (IRI) have been proposed, among them, the use of Cyclosporine A (CsA). However, studies present controversial results in relation to its benefits. The goal of this study was asses the effect of CsA in kidney IRI in rats. This is an experimental study conducted from March to June of 2013 in the laboratory of Unidade de Pesquisa Experimental (UNIPEX) of UNESP in Botucatu. Variables of homeostasis: systolic blood pressure (PAS), diastolic blood pressure (PAD), mean blood pressure (PAM), weight and temperature (T) controls were included, as were the measures of serum creatinine, urea nitrogen, and sodium, and RIFLE classification (protocol of diagnosis and risk stratification in acute renal injury), measured during three moments: PAS, PAD, PAM and T were measured at T0 (after catheterization of the carotid artery); T1 (after the clamping of the left renal artery); T2 (30 minutes after reperfusion). Serum creatinine, urea nitrogen and sodium were collected at M0 (T0); M1 (T2) and M2, 12 hours after the initial surgical procedure. Histological evaluation was performed at the end of the experiment. Thirty rats were included, randomly allocated in three groups: sham group (GS), which had only laparotomy and right nephrectomy; control group (GC) which additionally had ischemia reperfusion maneuver, and cyclosporine A group (GCsA), which besides having the afore mentioned procedures would use CsA intraperitoneal (15 mg/kg), 24 hours before and at the moment of initial surgical procedure. The RIFLE descriptive evaluations showed that the Control group and the CsA group presented negative results in relation to the Sham group. The assessment of the sodium levels did not present a meaningful difference of its numbers in each group during the three moments. About kidney function, the paired ...