Avaliação de métodos de coleta, centrifugação e manejo pré-coleta de fezes em estudos de digestibilidade para o tambaqui

• Ano de defesa: 2016
• Autor(a) principal: Assis, Rudney Weiber Silva de [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Peixe; Tambaqui (Peixe); Dissecação; Peixe - Alimentação; Digestão; Fezes; Fishes
• Link de acesso: http://hdl.handle.net/11449/143061

The study aimed to obtain information on methods of collection and processing of feces to prepare a protocol on protein and energy digestibility studies for tambaqui with average weight of 230,74g, by determining the best food pre-collection of stool frequency; assessing the effect of spin tambaqui feces and determining the best time to collect feces. The test of adequacy of food frequency was divided into daytime phase (fishes fed with different frequencies: 08:00 and 18:00, 8:00, 13:00 and 18:00, 08:00, 11:20, 14:40 and 18:00) and night phase (20:00 and 06:00, 20:00, 01:00 and 06:00, 20:00, 23:20, 02:40 and 06:00). In both cases (daytime and nighttime), the feces were collected every two hours. The evaluation parameter used to determine the best feed rate was the amount of stool in the dry matter. The testo f the effect of centrifugation was performed by determining the best for determining the best time and feces spin speed was lined with nine treatments following a factorial 3 X 3 comprises three rotation speeds (3000, 4500 and 6000 RPM) and three centrifugation time of stool (10, 20, and 30'), plus two treatments witnesses: feces collection by decantation without centrifuging and collecting stool by dissecting the final region of the digestive tract. The evaluation parameter used to determine the best response was the concentration of the marker protein and energy as well as its digestibility. For testing stool collection intervals were considered five treatments: 1h; 2h; 4h; 8h and 12h, with three replications. The best answer evaluation parameter was the concentration of protein and energy in the stool, and also their digestibility. It was concluded that there was no effect of different feeding frequencies on the volume of dry matter of feces, possibly tambaqui's ability to store food for a relatively long time. In general, no greater accuracy in collecting stool with centrifugation, and may be observed loss coefficients ...