Estudo da interação entre a via genética do microRNA156/squamosa promoter-binding protein-like e brassinosteróides durante o desenvolvimento de Arabidopsis thaliana

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Rojas, Carlos Hernán Barrera [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/128123
Resumo: Plant development is affected by different factors such as micro-RNAs (miRNAs) and phytohormones which interact in a complex regulation network. Among miRNAS, miRNA156 (miR156) regulates SQUAMOSA Promoter- Binding Protein-Like (SPL) transcription factor family affecting different plant development processes. Among phytohormones, brassinosteroids (BRs) participate in regulation of vegetal juvenile processes. miR156/SPL-BRs interaction is unknown whereby the aim of this work was to evaluate the interaction between those two pathways during Arabidopsis thaliana juvenile development. Main root (RP), lateral root number (RL) and hypocotyl length were selected as markers of this interaction. A. thaliana (Col-0 ecotype) overexpressing miR156 (miR156-OE), plants with miR156 reduced activity (Mimicry-156) and wild type (WT) plants were used. 24-Epibrassinolide (24- EBL), the most active BRs, was selected. miR156-OE plants have longer RP length, more RL and 24-EBL sensitivity. Opossite phenotypes were observed on Mimicry-156 plants. Besides, miR156-OE plants have longer hypocotyl length while Mimicry-156 plants have shorter. SPLs genes, SPL2, -3, -4, -5, and -6 responded to 24-EBL treatment. BRs pathway genes, CPD, BZR1, BES1 and BAS1 had changes in gene expression. Our data suggest a interaction between the miR156/SPL and BRs pathways and help to understand the molecular genetics of Arabidopsis development