Efeitos da sexagem por citometria de fluxo sobre a composição protéica da membrana de espermatozóides bovinos

• Ano de defesa: 2014
• Autor(a) principal: Scott, Caroline [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Bovino - Reprodução; Citometria de fluxo; Proteinas da membrana; Bovino - Semen; Celulas; Cattle Reproduction
• Link de acesso: http://hdl.handle.net/11449/108796

The sexing of sperm, to obtain doses of semen with a high percentage of gametes carrying the X or Y chromosome, even more relevant the role of artificial insemination in maximizing the genetic progress between generations, productivity and economic returns in some farming activities; in assisted reproduction in mammals, and in genetic counseling of the human species. Several techniques are described in an attempt to separate the sperm by sex, but all are still in process and no fitness offers 100% accuracy. Based on the hypothesis that during the process of spermatogenesis there are proteins that are differently expressed in the sperm membrane carrying the X or Y chromosome the separation technique using sex-specific antibodies have been extensively studied. Thus this work aimed to study membrane proteins expressed in sexed sperm and not sexed