Estudo da interação da natamicina com membranas fosfolipídicas incorporadas de esteróis

• Ano de defesa: 2014
• Autor(a) principal: Arima, Anderson Akira [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Antimicóticos; Colesterol; Filmes finos multifolhados; Lipossomos; Antifungal agents
• Link de acesso: http://hdl.handle.net/11449/110890

Natamycin is a broad spectrum antifungal, that due to its low oral and topical absorption, it already has been used both for food preservative and in the treatment of superficial mycoses. Althrough this natural antibiotic has been from soil bacteria of genus Stremptomyces since 1950, its mechanism of action on lipid environment of biomembranes has not been investigated enough. For this reason, in this study we investigated the effects of this antibiotic on two types of lipid model membranes - Langmuir films (monolayers) and multilamelar vesicles (liposomes) - and the obtained results were correlated with its mode of action on biomembranes. For monolayer models, the effect of natamycin on their mechanical properties, morphology and molecular organization were analyzed by, respectively, surface pressure isotherms, Brewsler angle Microscopy (BAM) and Polarization-Modulation Infrared Reflection-Absorption Spectroscopy (PM-IRRAS); while the influence of natamycin on the lipid organization and the thermal properties for liposomes were characterized by, respectively, Electron Paramagnetic Resonance (EPR) and Differential Scanning Calorimetry (DSC)